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Effects of Mycoplasma Elimination Agent on Maintenance and Cardiogenic Potentials of Human Embryonic Stem Cells

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Authors
Kim, Yoon Young; Ku, Seung-Yup; Kim, Yong Jin; Cho, Myung Soo; Kim, Seok Hyun; Choi, Young Min; Moon, Shin Yong; Oh, Sun Kyung
Issue Date
2010-10
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE; Vol.7(4); 419-424
Keywords
human embryonic stem cellsmycoplasmacardiogenic potenlialantibiotics
Abstract
Human embryonic stem cells (hESCs) are an attractive cell source for cell-based therapy considering their unique characteristics of self-renewal and pluripotency. Optimal culture condition of hESCs is important for the continuous maintenance and differentiation of hESCs. For the elimination or prevention of mycoplasma contamination, antibiotics are used for routine culture of various cell lines, however, their effects on the differentiation of hESCs were not clarified. In this study, we tried to demonstrate the effects of mycoplasma removal agent, plasmocin, on undifferentiated hESCs and derived differentiating cells. There were no significant effects on the morphology and the expression of Oct4, Sox2 and Nanog in undifferentiated hESCs. However, cardiogenic potential of hESCs were significantly affected. The expression of cardiac lineage markers such as Nkx2.5, cTn I and Tbx5 was down-regulated in plasmocin group. No dose-response relationship was observed. These results imply that differentiation of hESCs into cardiac lineage can be affected by antibiotics. Therefore, careful selection of infection prevention agent is necessary.
ISSN
1738-2696
Language
English
URI
http://hdl.handle.net/10371/78592
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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