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HBx modulates iron regulatory protein 1-mediated iron metabolism via reactive oxygen species

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dc.contributor.authorGu, Jin-Mo-
dc.contributor.authorLim, Seung Oe-
dc.contributor.authorOh, Sae Jin-
dc.contributor.authorYoon, So-Mi-
dc.contributor.authorSung, Je Kyeong-
dc.contributor.authorJung, Guhung-
dc.date.accessioned2009-08-26T22:39:23Z-
dc.date.available2009-08-26T22:39:23Z-
dc.date.issued2008-02-08-
dc.identifier.citationVirus Res. 133 (2), 167-177en
dc.identifier.issn0168-1702-
dc.identifier.urihttp://hdl.handle.net/10371/7886-
dc.description.abstractHepatitis B virus X protein (HBx) is involved in viral metabolism and progression of liver disease. Iron metabolism plays a significant role in liver disease. In this report, to elucidate the relationship between iron metabolism and HBx, we established the Huh7 cell lines in which HBx was stably expressed (Huh7-HBx). In Huh7-HBx, we observed that transferrin receptor 1 (TfR1) expression decreased and ferritin heavy chain (FtH) expression increased as well as reactive oxygen species (ROS) level increased. We also found that these modulations were caused by the downregulation of iron regulatory protein 1 (IRP1). Furthermore, the levels of total iron and labile iron pool (LIP) were altered in Huh7-HBx. In addition, antioxidant N-acetylcystein (NaC) increased IRP1 expression by depleting HBx-induced ROS. We also confirmed these alterations of TfR1 and FtH in the primary hepatocytes of HBx transgenic mice and in HepG2.2.15 cells that constitutively replicate the intact HBV genome. In conclusion, these results suggest that HBx modulates iron metabolism via ROS leading to pathological status in liver diseases.en
dc.description.sponsorshipThis study was supported by a grant from the National R&D
Program for Cancer Control, the Ministry of Health & Welfare,
Korea (0520020).
Jin-Mo Gu and Seung Oe Lim were supported by BK21
Research Fellowship from the Ministry of Education and Human
Resources Development.
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectIronen
dc.subjectHBxen
dc.subjectROSen
dc.subjectIRP1en
dc.subjectTotal iron levelen
dc.subjectLIPen
dc.titleHBx modulates iron regulatory protein 1-mediated iron metabolism via reactive oxygen speciesen
dc.typeArticleen
dc.contributor.AlternativeAuthor구진모-
dc.contributor.AlternativeAuthor임승외-
dc.contributor.AlternativeAuthor오새진-
dc.contributor.AlternativeAuthor윤소미-
dc.contributor.AlternativeAuthor성제경-
dc.contributor.AlternativeAuthor정구홍-
dc.identifier.doi10.1016/j.virusres.2007.12.014-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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