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In vivo alternative testing with zebrafish in ecotoxicology

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Authors
Seok, Seung-Hyeok; Baek, Min-Won; Lee, Hui-Young; Kim, Dong-Jae; Na, Yi-Rang; Noh, Kyoung-Jin; Park, Sung-Hoon; Lee, Hyun-Kyoung; Lee, Byoung-Hee; Park, Jae Hak
Issue Date
2008
Publisher
대한수의학회 = The Korean Society of Veterinary Science
Citation
J Vet Sci 2008, 9, 351-357
Keywords
aryl hydrocarbon receptorenhanced green fluorescentzebrafish
Abstract
Although rodents have previously been used in
ecotoxicological studies, they are expensive, time-consuming,
and are limited by strict legal restrictions. The present
study used a zebrafish (Danio rerio) model and generated
data that was useful for extrapolating toxicant effects in
this system to that of humans. Here we treated embryos of
the naive-type as well as a transiently transfected
zebrafish liver cell line carrying a plasmid (phAhREEGFP),
for comparing toxicity levels with the well-known
aryl hydrocarbon receptor (AhR)-binding toxicants:
3,3’,4,4’,5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-
p-dioxin, and 3-methylcholanthrene. These
toxicants induced a concentration-dependent increase in
morphological disruption, indicating toxicity at early
life-stages. The transient transgenic zebrafish liver cell
line was sensitive enough to these toxicants to express the
CYP1A1 regulated enhanced green fluorescent protein.
The findings of this study demonstrated that the zebrafish
in vivo model might allow for extremely rapid and
reproducible toxicological profiling of early life-stage
embryo development. We have also shown that the
transient transgenic zebrafish liver cell line can be used
for research on AhR mechanism studies.
ISSN
1229-845X
Language
English
URI
http://www.vetsci.org/2008/abstract/351a.html

http://hdl.handle.net/10371/7899
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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