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Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device

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Authors
Jeon, Noo Li; Baskaran, Harihara; Dertinger, Stephan K. W.; Whitesides, George M.; Van De Water, Livingston; Toner, Mehmet
Issue Date
2002-07-01
Publisher
Nature Publishing Group
Citation
Nature Biotechnology 20, 826-830 (2002)
Keywords
chemotaxismicrofluidic devicegradient
Abstract
Although a wealth of knowledge about chemotaxis has accumulated in the past 40 years, these studies have been hampered by the inability of researchers to generate simple linear gradients instantaneously and to maintain them at steady state. Here we describe a device microfabricated by soft lithography and consisting of a network of microfluidic channels that can generate spatially and temporally controlled gradients of chemotactic factors. When human neutrophils are positioned within a microchannel, their migration in simple and complex interleukin-8 (IL-8) gradients can be tested. The cells exhibit strong directional migration toward increasing concentrations of IL-8 in linear gradients. Neutrophil migration halts abruptly when cells encounter a sudden drop in the chemoattractant concentration to zero ("cliff" gradient). When neutrophils are challenged with a gradual increase and decrease in chemoattractant ("hill" gradient), however, the cells traverse the crest of maximum concentration and migrate further before reversing direction. The technique described in this paper provides a robust method to investigate migratory cells under a variety of conditions not accessible to study by earlier techniques.
ISSN
1087-0156
Language
English
URI
http://hdl.handle.net/10371/7976
DOI
https://doi.org/10.1038/nbt712
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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