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Neutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devices

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dc.contributor.authorLin, Francis-
dc.contributor.authorNguyen, Connie Minh-Canh-
dc.contributor.authorWang, Shur-Jen-
dc.contributor.authorSaadi, Wajeeh-
dc.contributor.authorGross, Steven P.-
dc.contributor.authorJeon, Noo Li-
dc.date.accessioned2009-08-28T05:09:55Z-
dc.date.available2009-08-28T05:09:55Z-
dc.date.issued2005-04-
dc.identifier.citationAnn. Biomed. Eng. 33:475-482, 2005en
dc.identifier.issn0090-6964 (print)-
dc.identifier.issn1573-9686 (online)-
dc.identifier.urihttp://hdl.handle.net/10371/7988-
dc.description.abstractNeutrophils migrating in tissue respond to complex overlapping signals generated by a variety of chemotactic factors (CFs). Previous studies suggested a hierarchy between bacteriaderived CFs and host-derived CFs but could not differentiate neutrophil
response to potentially equal host-derived CFs (IL-8 and LTB4). This paper reports neutrophil migration in conflicting gradients
of IL-8 and LTB4 using a microfluidic chemotaxis device that can generate stable and well-defined gradients. We quantitatively
characterized the movement of cells from time-lapse images. Neutrophils migrate more efficiently toward single IL-8 gradients than single LTB4 gradients as measured by the effective chemotactic index (ECI). In opposing gradients of IL-8 and LTB4,
neutrophils show obvious chemotaxis toward a distant gradient, consistent with previous reports. When an opposing gradient of
LTB4 is present, neutrophils show less effective chemotaxis toward IL-8 than when they are in a gradient of IL-8 alone. In
contrast, the chemotactic response of neutrophils to LTB4 is not reduced in opposing gradients as compared to that in a single LTB4 gradient. These results indicate that the presence of one host-derived CF modifies the response of neutrophils to a second CF suggesting a subtle hierarchy between them.
en
dc.description.sponsorshipF. Lin thanks the National Institutes of Health General
Medical Science Grant (GM-66051) for a fellowship.
We thank the excellent service provided by the General
Clinical Research Center (GCRC) at UC Irvine. This research
was supported by NSF (DBI-0138055) and NIH
(R03AIO055033).
en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.subjectChemotaxisen
dc.subjectGradienten
dc.subjectHierarchyen
dc.subjectMicrofluidicen
dc.subjectMigrationen
dc.titleNeutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devicesen
dc.typeArticleen
dc.contributor.AlternativeAuthor전누리-
dc.identifier.doi10.1007/s10439-005-2503-6-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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