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Engineering of a multi-functional extracellular matrix protein for immobilization to bone mineral hydroxyapatite
Cited 7 time in
Web of Science
Cited 11 time in Scopus
- Authors
- Issue Date
- 2011-01
- Publisher
- SPRINGER
- Citation
- BIOTECHNOLOGY LETTERS, Vol.33, No.1, pp.199-204
- Keywords
- Bone grafting and repair ; Hydroxy apatite ; Osteocalcin ; Fibronectin ; Cell adhesion ; Extracellular matrix
- Abstract
- Purpose of Work We have developed a strategy of designing multi-functional extracellular matrix proteins for functionalizing bone tissue engineering scaffolds and other biomedical surfaces to achieve improvements in bone grafting, bone repair and bone regeneration. We developed a novel extracellular matrix protein designed to have a cell adhesive RGD sequence derived from fibronectin and active functional unit of osteocalcin (OC) containing Ca2(+)-binding sites for immobilization to mineral component of bone, hydroxyapatite (HA). The fusion protein, designated FN(RGD)/OC, was expressed in Escherichia coli and purified with affinity chromatography using a His-tag. The resultant FN(RGD)/OC fusion protein preferentially bound to HA, promoted cell adhesive activity, and stimulated differentiation of MC3T3-E1 cell.
- ISSN
- 0141-5492
- Language
- English
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