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Human feeder layer system derived from umbilical cord stromal cells for human embryonic stem cells

Cited 15 time in Web of Science Cited 16 time in Scopus
Authors

Cho, Meeyoung; Lee, Eun Ju; Nam, Hyun; Yang, Ji-Hye; Lim, Jeong Mook; Lee, Gene; Cho, Jaejin

Issue Date
2010-05-15
Publisher
ELSEVIER SCIENCE INC
Citation
FERTILITY AND STERILITY, Vol.93, No.8, pp.2525-2531
Keywords
Human umbilical cord stromal cellshuman embryonic stem cellsserum free culture
Abstract
Objective: To evaluate the potential of human umbilical cord-derived stromal cells (hUCSCs) as a human feeder for human embryonic stem cells (ESCs). Design: Prospective study. Setting: Laboratory of Molecular Genetics and Stem Cell Differentiation, Dental Research Institute, School of Dentistry, Seoul National University. Intervention(s): The hUCSCs were established, and human ESCs were cultured on established hUCSCs without serum. Main Outcome Measure(s): Cell-surface markers, karyotyping, and teratoma formation. Result(s): Primary cultures of hUCSCs from individual umbilical cords were maintained by an established protocol. Human ESCs on hUCSC layers were successfully maintained in serum-free culture medium past passage 30. Compared with hESCs on mouse feeder cells, the hESCs on hUCSCs showed similar levels of pluripotency-related cell-surface markers, self-renewal capacity, and teratoma formation in immune-deficient mice. These ESCs cultured on hUCSCs had a normal karyotype, even after long-term culture. Conclusion(s): The hUCSCs supported self-renewal of hESCs in serum-free conditions. This culture system has the potential to facilitate the development of clinical-grade hESCs for regenerative medicine. (Fertil Steril (R) 2010; 93: 2525-31. (C) 2010 by American Society for Reproductive Medicine.)
ISSN
0015-0282
Language
English
URI
https://hdl.handle.net/10371/80377
DOI
https://doi.org/10.1016/j.fertnstert.2010.03.027
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