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CXCR-4 knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells

Cited 22 time in Web of Science Cited 23 time in Scopus
Authors
Kim, Ji-Hong; Lee, Jae-Il; Hong, Sam-Pyo; Hong, Seong-Doo; Kim, Mi-Ae; Pai, Hyun-Kyung; Hong, Kyoung-Ok; Hong, Ji-Soo
Issue Date
2009-02
Publisher
WILEY-BLACKWELL PUBLISHING, INC
Citation
JOURNAL OF ORAL PATHOLOGY & MEDICINE, Vol.38, No.2, pp.214-219
Keywords
CXCR-4 knockdowninvasionproliferationsiRNAoral squamous cell carcinoma
Abstract
Oral squamous cell carcinomas (OSCCs) are characterized by a high degree of local invasion and a high rate of metastases to cervical lymph nodes. Downregulation of CXCR-4 by siRNA inhibits invasion and growth of breast and colon cancer cells. However, there have been no reports on the downregulation of CXCR-4 by small interfering RNA (siRNA) in oral cancer cells. We generated two stable CXCR-4-knockdown clones (KBsi and KOSCC-25Bsi) from the KB and KOSCC-25B OSCC cell lines by lentiviral delivery. In vitro invasion and cell proliferation assays were used to investigate the effect of CXCR-4 downregulation on cell proliferation and invasiveness in KBsi and KOSCC-25Bsi. Immunohistochemistry was performed to evaluate the correlation between CXCR-4 expression and proliferation in 26 OSCC tissue samples. CXCR4-knockdown OSCC cells showed reduced invasiveness. The invasiveness of KBsi decreased to 29.5% of the vector-infected controls, and KOSCC-25Bsi decreased to 38.1% of the control vector-infected cells (P < 0.05). The CXCR4-knockdown OSCC cells grew significantly slower than the vector-infected control cells. KBsi and KOSCC-25Bsi cells proliferated at 69.5% and 71.7%, respectively, of the rate of control vector-infected cells (P < 0.05). CXCR-4-positive group had significantly higher PCNA labeling index than CXCR-4-negative group in OSCC tissue samples. These results suggest that the downregulation of CXCR-4 induces anti-proliferative and anti-invasive effects in OSCC and that CXCR-4 might be a useful target molecule for the treatment of OSCC.
ISSN
0904-2512
Language
English
URI
http://hdl.handle.net/10371/80392
DOI
https://doi.org/10.1111/j.1600-0714.2008.00671.x
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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