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Identification of Porphyromonas gingivalis lipopolysaccharide-binding proteins in human saliva

Cited 28 time in Web of Science Cited 30 time in Scopus
Authors
Choi, Seulggie; Baik, Jung Eun; Jeon, Jun Ho; Cho, Kun; Kum, Kee-Yeon; Han, Seung Hyun; Yun, Cheol-Heui; Seo, Deog-Gyu
Issue Date
2011-09
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
MOLECULAR IMMUNOLOGY, Vol.48, No.15-16, pp.2207-2213
Keywords
Porphyromonas gingivalisLipopolysaccharideLipopolysaccharide-binding proteinsMass spectrometrySaliva
Abstract
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified Pg.LPS-binding proteins (Pg.LPS-BPs) using Pg.LPS-immobilized beads and a high-resolution mass spectrometry. LPS purified from P. gingivalis was conjugated onto N-hydroxysuccinimidyl-Sepharose (R) 4 Fast Flow beads. Notably, Pg.LPS-conjugated beads could stimulate Toll-like receptor 2 (TLR2) as determined by a TLR2-depdendent reporter expression system using CHO/CD14/TLR2. In addition, the Pg.LPS-conjugated beads induced the production of inflammatory mediators such as nitric oxide and interferon-gamma-inducible protein-10 in the macrophage cell-line, RAW 264.7. These results imply that Pg.LPS retained its immunological properties during the conjugation process. Then, the Pg.LPS-conjugated beads were mixed with a pool of saliva obtained from nine human subjects to capture Pg.LPS-BPs and molecular identities were determined by LTQ-Orbitrap hybrid fourier transform mass spectrometry. Pg.LPS-BPs captured at high frequencies included alpha-amylase, cystatin, prolactin-inducible protein, lysozyme C, immunoglobulin components, serum albumin, lipocalin-1, and submaxillary gland androgen-regulated protein 3B. These proteins are known to be involved in bacterial adhesion and colonization, anti-microbial functions or modulation of immune responses. (C) 2011 Elsevier Ltd. All rights reserved.
ISSN
0161-5890
Language
English
URI
http://hdl.handle.net/10371/80470
DOI
https://doi.org/10.1016/j.molimm.2011.06.434
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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