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Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells

Cited 353 time in Web of Science Cited 371 time in Scopus
Authors
Hwang, Soon Jung; Tae, Giyoong; Noh, Insup; Lee, Sang Hoon; Park, Yongdoo; Kim, In Sook; Lee, Kyu Back; Cho, Tae Hyung; Kim, Jungju; Sun, Kyung
Issue Date
2007-04
Publisher
ELSEVIER SCI LTD
Citation
BIOMATERIALS; Vol.28, No.10, pp.1830-1837
Keywords
hyaluronic acidhydrogelbone morphogenic proteinmesenchymal stem cellsbone regeneration
Abstract
Acrylated hyaluronic acid (HA) was used as a scaffold for bone morphogenic protein-2 (BMP-2) and human mesenchymal stem cells (hMSCs) for rat calvarial defect regeneration. HA was acrylated by two-step reactions: (1) introduction of an amine group using adipic acid dihydrazide (ADH); (2) acrylation by N-acryloxysuccinimide. Tetrathiolated poly(ethylene) glycol (PEG-SH4) was used as a crosslinker by a Michael-type addition reaction and the hydrogel was formed within 10 min under physiological conditions. This hydrogel is degraded completely by 100 U/ml hyaluronidase in vitro. hMSCs and/or BMP-2 was added during gelation. Cellular viability in vitro was increased up to 55% in the hydrogels with BMP-2 compared with the control. For in vivo calvarial defect regeneration, five different samples (i.e., control, hydrogel, hydrogel with BMP-2, hydrogel with MSCs, and hydrogel with BMP-2 and MSCs) were implanted for 4 weeks. The histological results demonstrated that the hydrogels with BMP-2 and MSCs had the highest expression of osteocalcin and mature bone formation with vascular markers, such as CD31 and vascular endothelial growth factors, compared with the other samples. This study demonstrated that HA base hydrogel can be used for cell and growth factor carriers for tissue regeneration. (c) 2006 Elsevier Ltd. All rights reserved.
ISSN
0142-9612
Language
English
URI
http://hdl.handle.net/10371/80504
DOI
https://doi.org/10.1016/j.biomaterials.2006.11.050
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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