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Intrathecal Administration of Botulinum Neurotoxin Type A Attenuates Formalin-Induced Nociceptive Responses in Mice

Cited 25 time in Web of Science Cited 28 time in Scopus
Authors
Lee, Won-Ho; Shin, Teo Jeon; Kim, Hyun Jeong; Lee, Jin-Koo; Lee, Sang Chul; Seo, Kyle; Suh, Hong-Won
Issue Date
2011-01
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Citation
ANESTHESIA AND ANALGESIA, Vol.112, No.1, pp.228-235
Description
This study was presented in part as a poster at the meeting of the 3rd WIP Congress in Barcelona, Spain (2004), and the 24th Annual Scientific Meeting of the American Pain Society in the United States (2005).
Abstract
BACKGROUND: Botulinum neurotoxin type A (BoNT/A) has been used as an analgesic for myofascial pain syndromes, migraine, and other types of headaches. Although an antinociceptive effect of central or peripheral administration of BoNT/A is suggested, the effect at the spinal level is still unclear. In this study, we evaluated the antinociceptive effect of intrathecally administered BoNT/A on the ICR mice during the formalin test. METHODS: BoNT/A (0.01 U/mouse) was injected intrathecally in ICR mice, and we observed formalin-induced inflammatory pain behaviors at days 1, 4, 7, 10, 14, 21, and 28 after the injection. We also examined the level of calcitonin gene-related peptide (CGRP), phosphorylated extracellullar signal-regulated kinases (p-ERK), and phosphorylated Ca(2+)/calmodulin-dependent protein kinase type 2 (p-CaMK-II) using immunoblot or immunohistochemical analyses before and after BoNT/A intrathecal injection. RESULTS: Even a single intrathecal injection of BoNT/A significantly decreased the nociceptive responses in the first phase (10 and 14 days later) and in the second phase of the formalin test at 1, 4, 7, 10, and 14 days later (P < 0.05) without any locomotor changes. Interestingly, intrathecal BoNT/A attenuated the expression level of CGRP, p-ERK, and p-CaMK-II in the 4th and 5th lumbar spinal dorsal horn at 10 days after injection in comparison with control. CONCLUSIONS: We showed that intrathecally administered BoNT/A may have a central analgesic effect on inflammatory pain through the modulation of central sensitization. BoNT/A, with its long-lasting antinociceptive effect, may be a useful analgesic in inflammatory pain. (Anesth Analg 2011;112:228-35)
ISSN
0003-2999
Language
English
URI
http://hdl.handle.net/10371/80543
DOI
https://doi.org/10.1213/ANE.0b013e3181ffa1d7
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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