녹농균증에 대한 다가 녹농균 백신효과의 실험적 연구

Abstract

Pseudomonas aeruginosa is a well-known nosocomial pathogen and chemotherapy of the pseudomonas infection is limited due to the intrinsic resistance of this organism to antimicrobial agents and to susceptibility of compromised hosts to this infection. To find out an effective immunological measure for the prevention and control of the pseudomonas infection, the frequency of Pseudomonas aeruginosa serotypes prevalent in the SNUH environment was surveyed and distribution of serological cross-reaction was determined. On the basis of the results obtained from the serological cross reactions, the 10 representative serotypes were divided into two polyvalent vaccines. And protective effect of the polyvalent vaccine were evaluated in the ICR mice. Following results were obtained. 1. Serotypes of 64 strains of Pseudomonsa aeruginosa isolated from SNUH ennvironment were as follow: serotype C were 14 (21. 9%), serotype B were 13(20.6%), serotype E were 10 OS. 696), serotype G were 7 00.9%), serotype A were 6 (9.496), serotype F were 3 (4.7%), serotype D were 2 (3.1%) and serotype 1 was 1 0.6%). 2. Heat killed whole cell polyvalent pseudomonas vaccine has protective effect against the infection induced experimentally by 12 serotypes of Pseudomonas aeruginosa. 3. Heat killed polyagglutinable pseudomonas vaccine has protective effect against the infection induced by only 7 serotypes of Pseudomonas aeruginosa and protective effect and agglutinin titer were not correlated.