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Intracellular degradation of Fusobacterium nucleatum in human gingival epithelial cells.

Cited 21 time in Web of Science Cited 20 time in Scopus
Authors
Ji, Suk; Shin, Ji Eun; Kim, Yong Cheol; Choi, Youngnim
Issue Date
2010
Publisher
Springer Verlag
Citation
Molecules and cells, Vol.30, No.6, pp.519-526
Keywords
endosomesfusobacterium nucleatumgingival epitheliuminvasionlysosomes
Abstract
The role of Fusobacterium nucleatum in oral health and disease is controversial. We have previously shown that F. nucleatum invades gingival epithelial cells. However, the destiny of the internalized F. nucleatum is not clear. In the present study, the intracellular destiny of F. nucleatum and its cytopathic effect on gingival epithelial cells were studied. The ability of F. nucleatum and seven other oral bacterial species to invade immortalized human gingival epithelial (HOK-16B) cells were compared by confocal microscopy and flow cytometry. F. nucleatum had the highest invasive capacity, comparable to that of Porphyromonas gingivalis, a periodontal pathogen. Confocal microscopic examination revealed colocalization of internalized F. nucleatum with endosomes and lysosomes. Examination by transmission electron microscopy revealed that most intracellular F. nucleatum was located within vesicular structures with single enclosed membranes. Furthermore, F. nucleatum could not survive within gingival epithelial cells and had no cytopathic effects on host cells. Interestingly, endosomal maturation played a role in induction of the antimicrobial peptides human beta defensin (HBD)-2 and -3 by F. nucleatum from gingival epithelial cells. F. nucleatum is destined to enter an endocytic degradation pathway after invasion and has no cytopathic effect on gingival epithelial cells, which may cast new light on the role of F. nucleatum in the pathogenesis of periodontitis.
ISSN
1016-8478
Language
English
URI
http://hdl.handle.net/10371/81057
DOI
https://doi.org/10.1007/s10059-010-0142-8
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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