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Intracellular degradation of Fusobacterium nucleatum in human gingival epithelial cells.

DC Field Value Language
dc.contributor.authorJi, Suk-
dc.contributor.authorShin, Ji Eun-
dc.contributor.authorKim, Yong Cheol-
dc.contributor.authorChoi, Youngnim-
dc.date.accessioned2013-01-23T08:25:40Z-
dc.date.available2013-01-23T08:25:40Z-
dc.date.issued2010-
dc.identifier.citationMolecules and cells, Vol.30, No.6, pp.519-526ko_KR
dc.identifier.issn1016-8478-
dc.identifier.urihttps://hdl.handle.net/10371/81057-
dc.description.abstractThe role of Fusobacterium nucleatum in oral health and disease is controversial. We have previously shown that F. nucleatum invades gingival epithelial cells. However, the destiny of the internalized F. nucleatum is not clear. In the present study, the intracellular destiny of F. nucleatum and its cytopathic effect on gingival epithelial cells were studied. The ability of F. nucleatum and seven other oral bacterial species to invade immortalized human gingival epithelial (HOK-16B) cells were compared by confocal microscopy and flow cytometry. F. nucleatum had the highest invasive capacity, comparable to that of Porphyromonas gingivalis, a periodontal pathogen. Confocal microscopic examination revealed colocalization of internalized F. nucleatum with endosomes and lysosomes. Examination by transmission electron microscopy revealed that most intracellular F. nucleatum was located within vesicular structures with single enclosed membranes. Furthermore, F. nucleatum could not survive within gingival epithelial cells and had no cytopathic effects on host cells. Interestingly, endosomal maturation played a role in induction of the antimicrobial peptides human beta defensin (HBD)-2 and -3 by F. nucleatum from gingival epithelial cells. F. nucleatum is destined to enter an endocytic degradation pathway after invasion and has no cytopathic effect on gingival epithelial cells, which may cast new light on the role of F. nucleatum in the pathogenesis of periodontitis.ko_KR
dc.language.isoenko_KR
dc.publisherSpringer Verlagko_KR
dc.subjectendosomesko_KR
dc.subjectfusobacterium nucleatumko_KR
dc.subjectgingival epitheliumko_KR
dc.subjectinvasionko_KR
dc.subjectlysosomesko_KR
dc.titleIntracellular degradation of Fusobacterium nucleatum in human gingival epithelial cells.ko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor지숙-
dc.contributor.AlternativeAuthor신지은-
dc.contributor.AlternativeAuthor김용철-
dc.contributor.AlternativeAuthor최영님-
dc.identifier.doi10.1007/s10059-010-0142-8-
dc.citation.journaltitleMolecules and cells-
dc.description.tc2-
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