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Regeneration of peripheral nerves by transplanted sphere of human mesenchymal stem cells derived from embryonic stem cells

DC Field Value Language
dc.contributor.authorLee, Eun Ju-
dc.contributor.authorXu, Lianji-
dc.contributor.authorKim, Gi-Hwan-
dc.contributor.authorKang, Soo Kyoung-
dc.contributor.authorLee, Sae-Won-
dc.contributor.authorPark, Sung-Hye-
dc.contributor.authorKim, Sukwha-
dc.contributor.authorChoi, Tae Hyun-
dc.contributor.authorKim, Hyo-Soo-
dc.creator박성혜-
dc.date.accessioned2013-03-21T09:53:49Z-
dc.date.available2013-03-21T09:53:49Z-
dc.date.issued2012-10-
dc.identifier.citationBIOMATERIALS Vol.33 No.29, pp. 7039-7046-
dc.identifier.issn0142-9612-
dc.identifier.urihttp://hdl.handle.net/10371/81386-
dc.description.abstractIn cell therapy, the most important factor for therapeutic efficacy is the stable supply of cells with best engraftment efficiency. To meet this requirement, we have developed a culture strategy such as three-dimensional sphere of human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) in serum-free medium. To investigate the in vivo therapeutic efficacy of hESC-MSC spheres in nerve injury model, we transected the sciatic nerve in athymic nude mice and created a 2-mm gap. Transplantation of hESC-MSC as sphere repaired the injured nerve significantly better than transplantation of hESC-MSC as suspended single cells in regard to 1) nerve conduction (sphere; 28.81 +/- 3.55 vs. single cells; 18.04 +/- 2.10, p < 0.05) and 2) susceptibility of nerve stimulation at low voltage (sphere; 0.38 +/- 0.08 vs. single cells; 0.66 +/- 0.11, p < 0.05) at 8 weeks. Recovery after sphere transplantation was near-complete when compared with the data of normal control (sphere 28.81 +/- 3.55 vs normal 32.62 +/- 2.85 in nerve conduction : sphere 0.38 +/- 0.08 vs normal 0.36 +/- 0.67 in susceptibility of nerve stimulation, no significant difference, respectively). Recovery in function of the injured nerve was well corroborated by the histologic evidence of regenerated nerve. In the mechanistic analysis, the supernatant of sphere-forming hESC-MSC contains hepatocyte growth factor and insulin-like growth factor-binding protein-1 significantly more than the supernatant of the single cells of hESC-MSC has, which might be the key factors for the improved engraftment efficiency and greater regeneration of injured peripheral nerve. (C) 2012 Elsevier Ltd. All rights reserved.en
dc.language.isoenen
dc.publisherELSEVIER SCI LTDen
dc.subject복합학en
dc.subjectSphere from hESC-MSCs-
dc.subjectPeripheral nerve injury-
dc.subjectCell transplantation-
dc.subjectSerum free-medium-
dc.titleRegeneration of peripheral nerves by transplanted sphere of human mesenchymal stem cells derived from embryonic stem cellsen
dc.typeArticle-
dc.contributor.AlternativeAuthor이은주-
dc.contributor.AlternativeAuthor김기환-
dc.contributor.AlternativeAuthor강수경-
dc.contributor.AlternativeAuthor이새원-
dc.contributor.AlternativeAuthor박성혜-
dc.contributor.AlternativeAuthor김석화-
dc.contributor.AlternativeAuthor최태현-
dc.contributor.AlternativeAuthor김효수-
dc.identifier.doi10.1016/j.biomaterials.2012.06.047-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2012-01/102/0000027894/11-
dc.description.srndSEQ:11-
dc.description.srndPERF_CD:SNU2012-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000027894-
dc.description.srndADJUST_YN:Y-
dc.description.srndEMP_ID:A075873-
dc.description.srndDEPT_CD:801-
dc.description.srndCITE_RATE:7.404-
dc.description.srndFILENAME:Lee-2012-Regeneration of peripheral nerves.pdf-
dc.description.srndDEPT_NM:의학과-
dc.description.srndEMAIL:shparknp@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.srnd2012-01/102/0000027894/11-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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