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Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
Cited 55 time in
Web of Science
Cited 61 time in Scopus
- Authors
- Issue Date
- 2012-03
- Publisher
- MDPI
- Citation
- MOLECULES Vol.17 No.3, pp. 2474-2490
- Keywords
- 복합학
; optically-encoded bead
; fluorescence
; quantum dots
; surface-enhanced Raman
scattering (SERS) ; bead-based assay ; label-free detection ; high-throughput screening
- Abstract
- Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
- ISSN
- 1420-3049
- Language
- English
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