토끼의 각 조직에 있어서 C14-포도당의 산화대사 경로의 비교에 관한 실험

Abstract

24 of rabbits were divided into 3 groups. e.g., the first group for liver incubation experiment, the second group for kidney incubation experiment, and the third group for brain incubation experiment. The same tissue homogenate obtained from each run of every group were subdivided into' 5 subgroups. Each subgroup was incubated for a period of 2 hours in the constant temperature water bath with incubation mixture consisting of 10 cc of phosphate buffer and 20 cc of oxygen. to which were added a glucose-10 4 in the subgroup "a", glucose-Ll-Cts in the subgroup "b", lactate-I-Cw in the subgroup "c", lactate-z-Ci- in the subgroup "d", and lactate-3-Cl4 in the subgroup "e", respectively. The gas samples obtained at the end of incubation period from each subgroup were analyzed for radioactivities of 0 402 produced. Data obtained from each groups were summerized as follow; 1) In the liver and kidney, C140 2 yields from glucose-LCw were always larger than that from glucose-U-Cl4, but in the brain tissues, Cl40 2 yields from glucose-Ll-Cw were generally larger than that from glucose-L-Cu, 2) In the lactate-Ct- incubation experiments, Cl402 yields from C-I of lactate (subgroup c) showed largest value as compared with those from C-2 and C-3 of lactate in each group. There were little differences in 0 40 2 yields from C-2 and C-3 of lactate in liver and kidney tissue. 3) Fractions of oxidative pathway of glucose were calculated from C1402 yields of 5 subgroups in each group. 32.8% of respiratory C02 was produced through EMp·TCA oxidative pathway from glucose in the first group, 59% in the second group and 96.8% in the third group.