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EGFR phosphorylation-dependent formation of cell-cell contacts by Ras/Erks cascade inhibition

Cited 11 time in Web of Science Cited 11 time in Scopus
Authors
Kang, Eun-Sil; Oh, Min-A; Lee, Sin-Ae; Kim, Tae Young; Kim, Sung-Hoon; Gotoh, Noriko; Kim, Yong-Nyun; Lee, Jung Weon
Issue Date
2007-06
Publisher
ELSEVIER
Citation
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH Vol.1773 No.6, pp. 833-843
Keywords
복합학cell-cell contactErkRasEGFR신호전달
Abstract
Cell-cell contacts play important roles in the homeostasis of normal epithelium and in the steps of metastasis of tumor cells, although signaling mechanisms to regulate cell-cell contacts are unclear. In this study, we observed that phenotype of no cell-cell contacts in rat intestinal epithelial cell subline (RIE1-Sca) correlated with increased Erk1/2 signaling activity, compared to that of parental RIE1 cells growing in colonies. Furthermore, cell-cell contacts between RIE1-Sca cells were reformed by treatment with a specific MEK inhibitor (U0126), with translocation of ZO1 and beta-catenin to cell-cell contacts, without changes of their expression levels. U0126 treatment also increased EGFR phosphorylation in a ligand-independent manner. Pretreatment with EGFR kinase inhibitor abolished U0126 treatment-mediated EGFR phosphorylation, and expression of dominant negative H-Ras N17 allowed EGFR phosphorylation and cell-cell contacts even without U0126 treatment. Furthermore, the expression of a nonphosphorylatable EGFR Y5F mutant abolished U0126-mediated cell-cell contacts. U0126 treatment also caused less efficient wound healing by keeping monolayer integrity intact, compared to control untreated cells. This U0126-mediated reduction in wound healing was further altered either by pretreatment of EGFR kinase inhibitor or expression of H-Ras N17 or EGFR Y517. Taken together, this study supports a unique mechanism of cell-cell contact formation through MEK/Erks inhibition-mediated EGFR phosphorylation. (c) 2007 Elsevier B.V. All rights reserved.
ISSN
0167-4889
Language
English
URI
http://hdl.handle.net/10371/82051
DOI
https://doi.org/10.1016/j.bbamcr.2007.02.003
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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