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Initial Osteoblast-like Cell Response to Titanium Coated with Recombinant Human Bone Morphogenic Protein-2 (rhBMP-2)

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Authors
Lee, Su Young; Koak, Jai Young; Heo, Seong Joo; Kim, Seong Kyun; Lee, Seung Jin; Shim, In Kyung
Issue Date
2010-03
Publisher
Korean Tissue Engineering and Regenerative Medicine Society
Citation
Tissue Engineering and Regenerative Medicine, Vol.7, No.1, pp. 64-68
Keywords
bone morphogenic protein (BMP)osteoblast-like cellPLGAtitanium
Abstract
The aim of this study was to investigate the initial bone cell response to titanium discs coated with a biodegradable polymer, poly(D,L-lactide-co-glycolide) (PLGA) incorporated recombinant human Bone Morphogenic Protein-2(rhBMP-2). Titanium discs were fabricated and divided into three groups. Group I was anodized under 300 V and not coated. Group 2 was machined and coated with PLGA/rhBMP-2 (500 ng/ml) solutions. Group 3 was anodized then coated with the same solution. Surface topography was examined by scanning electron microscopy (SEM). The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured. MTS based cell proliferation assay for 1, 3 days and alkaline phosphatase activity test were carried out for 3 and 7 days. It was observed that PLGA/rhBMP particles were observed as smooth and round onto the coated titanium surfaces. Cell proliferation and ALP activity were the highest on anodized and coated Ti surfaces (group 3). In this study, biodegradable PLGA polymers incorporated with rhBMP could enhance proliferation and differentiation of cells on the titanium surface.
ISSN
1738-2696
Language
English
URI
http://www.term.or.kr/journal/view.php?number=504

http://hdl.handle.net/10371/82363
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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