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Effects of calcium phosphate endodontic sealers on the behavior of human periodontal ligament fibroblasts and MG63 osteoblast-like cells
Cited 9 time in
Web of Science
Cited 12 time in Scopus
- Authors
- Issue Date
- 2012-11
- Publisher
- WILEY-LISS
- Citation
- JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS Vol.100B No.8, pp. 2141-2147
- Keywords
- 의약학
; CAPSEAL
; calcium phosphate-based root canal
sealer ; human periodontal fibroblast cells ; inflammatory mediator ; osteoblast differentiation
- Abstract
- In regard to biological properties of endodontic
sealers, there are many characteristics that should be considered. The aim of this study was to examine the biological
effects of new calcium phosphate-based root canal sealers,
CAPSEAL I and CAPSEAL II (CPS), on human periodontal
fibroblast cells by examining the expression levels of inflammatory mediators and to compare the effects of CPS on the
viability and osteogenic potential of human osteoblast MG63
cells compared to those of other commercially available calcium phosphate sealers [Apatite Root Sealer type I (ARS I)
and Apatite Root Sealer III (ARS III); Sankin Kogyo, Tokyo, Japan] and a zinc oxide eugenol-based sealer (Pulp Canal
Sealer EWT [PCS EWT]; Kerr, Detroit, MI). The levels of IL-6
in the new CPS group (CAPSEAL I, II) were higher than those
in the control and all experimental groups at all time points
after 2 h. TGF-b1 and FGF-1 levels decreased at 72 h compared to the levels in the control, in cells treated with every
sealers except ARS I. The new CPS sealers showed low cytotoxicity. Reverse transcription polymerase chain reaction
showed that CAPSEAL I, II, and Apatite Root Sealer type III
induced expression of early stage markers of differentiation
(alkaline phosphatase and osteopontin) at 7 days. Also, new
CPS showed higher mineralized nodule formation at 28 days.
These results suggest that CAPSEAL I and II facilitate the
periapical dentoalveolar and alveolar healing by controlling
cellular mediators from PDL cells and osteoblast differentiation of precursor cells.
- ISSN
- 1552-4973
- Language
- English
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