담즙의 화학적 및 약리학적 연구

Abstract

Extracts was obtained from the sap flowing from the stump of Rhus Verniciflua Stokes by using alcohoI and ether as solvents. Urushiol was obtained by distilling this obtained extract fractionally. The following materials are separated from urushiol through the means of Aluminium column chromatography by which mixture solvent (ethylether, ethyl alcohol and chlorform) was used: a) Triolefinic component(A fraction), which has 3 double bonds. b) Diolefinic component(B fraction), which has 2 double bonds. c) Monoolefinic component(C fraction), which has 1 double bond. d) Hydrourushiolr D fraction), which is fully saturated. These materials were chemically confirmed, and B fraction was converted to Mehtyl ether to compare with free hydroxyl group in B fraction. The following experiments were done in order to study the relationship between the above said chemical structures and physiological means. These experiments were done under the consideration that the nucleous of urushiol, which is used as Anthelmintics among the country people, is homologue to with of Hexylresorci nol. These experiments were on anthelmintic and general pharmacological actions of urushiol. And the tissue reaction phenomena intensity and toxicity of each fraction of urushiol were comparatively experimented. The intensity of tissue reaction phenomena increased in proportion the increase of the double bonds of the side chain of urushiol, but its toxicity decreased on urushiol. Hydroxyl radical in the nucleous of urushiol influenced a little on the intensity of action, not on the physiological actions. Accordingly the effective factor is the side chain and the double bonds in the side chain.