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Detection by In-situ Hybridization of Pasteurella multocida Toxin (toxA) Gene in the Lungs of Naturally Infected Pigs

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Authors
Ahn, K.K.; Lee, Y.H.; Ha, Y.; Kim, D.; Chae, S.; Kim, C.H.; Lee, J.-H.; Kim, S.-H.; Chae, Chan Hee
Issue Date
2008-05-20
Publisher
Elsevier
Citation
Journal of Comparative Pathology, 139, 51-53
Keywords
bacterial infectionPasteurella multocidapigpulmonary abscesstoxA gene
Abstract
In-situ hybridization with a non-radioactive digoxigenin-labelled probe was used to detect the Pasteurella multocida toxin (PMT) gene in tissue sections of pneumonic lung from pigs naturally infected with toxigenic P. multocida. The morphology of host cells was preserved despite the relatively high temperature used in the incubation procedure. Pulmonary abscessation was observed in 13 pigs naturally infected with toxigenic P. multocida type A (three pigs) or D (10 pigs). In these 13 pigs a strong hybridization signal for PMT DNA was detected, mainly in degenerate leucocytes in abscesses. Occasionally, PMT DNA was detected in degenerate neutrophils and macrophages in alveolar spaces. Detection of hybridization signals for PMT DNA would seem to be a potential indicator of the production of PMT. The study suggested that PMT plays an important role in pulmonary abscessation caused by P. multocida.
ISSN
0021-9975
Language
English
URI
http://hdl.handle.net/10371/8276
DOI
https://doi.org/10.1016/j.jcpa.2008.03.002
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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