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Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells

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Authors
Park, Eun-Jung; Yi, Jongheop; Chung, Kyu-Hyuck; Ryu, Doug Young; Choi, Jinhee; Park, Kwangsik
Issue Date
2008-07-10
Publisher
Elsevier
Citation
Toxicol. Lett. 180, 222-229
Keywords
Titanium dioxide nanoparticlesApoptosisOxidative stressBEAS-2B cells
Abstract
As the applications of industrial nanoparticles are being developed, the concerns on the environmental
health are increasing. Cytotoxicities of titanium dioxide nanoparticles of different concentrations (5,
10, 20 and 40 g/ml) were evaluated in this study using a cultured human bronchial epithelial cell line,
BEAS-2B. Exposure of the cultured cells to nanoparticles led to cell death, reactive oxygen species (ROS)
increase, reduced glutathione (GSH) decrease, and the induction of oxidative stress-related genes such
as heme oxygenase-1, thioredoxin reductase, glutathione-S-transferase, catalase, and a hypoxia inducible
gene. The ROS increase by titanium dioxide nanoparticles triggered the activation of cytosolic caspase-
3 and chromatin condensation, which means that titanium dioxide nanoparticles exert cytotoxicity by
an apoptotic process. Furthermore, the expressions of inflammation-related genes such as interleukin-
1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), TNF-a, and C-X-C motif ligand 2 (CXCL2) were also
elevated. The induction of IL-8 by titanium dioxide nanoparticles was inhibited by the pre-treatment
with SB203580 and PD98059, which means that the IL-8 was induced through p38 mitogen-acitvated
protein kinase (MAPK) pathway and/or extracellular signal (ERK) pathway. Uptake of the nanoparticles
into the cultured cells was observed and titanium dioxide nanoparticles seemed to penetrate
into the cytoplasm and locate in the peri-region of the nucleus as aggregated particles, which may
induce direct interactions between the particles and cellular molecules, to cause adverse biological
responses.
ISSN
0378-4274
Language
English
URI
http://hdl.handle.net/10371/8291
DOI
https://doi.org/10.1016/j.toxlet.2008.06.869
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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