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Molecular characterization of T-type Ca2+ channels responsible for low threshold spikes in hypothalamic paraventricular nucleus neurons

Cited 19 time in Web of Science Cited 18 time in Scopus
Authors
Lee, S.; Han, T.H.; Sonner, P.M.; Stern, J.E.; Ryu, Pan Dong; Lee, S.Y.
Issue Date
2008-07-03
Publisher
Elsevier
Citation
Neuroscience 155:1195-1203
Keywords
preautonomic neuronssingle cell RT-PCRretrograde labelingslice patch clampCav3.1nickel
Abstract
The hypothalamic paraventricular nucleus (PVN) is composed of functionally heterogeneous cell groups, possessing distinct electrophysiological properties depending on their functional roles. Previously, T-type Ca2+ dependent low-threshold spikes (LTS) have been demonstrated in various PVN neuronal types, including preautonomic cells. However, the molecular composition and functional properties of the underlying T-type Ca2+ channels have not been characterized. In the present study, we combined single cell reverse transcription–polymerase chain reaction (RT-PCR), immunohistochemistry and patch-clamp recordings to identify subtypes of T-type Ca2+ channels expressed in PVN cells displaying LTS (PVN-LTS), including identified preautonomic neurons. LTS appeared at the end of hyperpolarizing pulses either as long-lasting plateaus or as short-lasting depolarizing humps. LTS were mediated by rapidly activating and inactivating T-type Ca2+ currents and were blocked by Ni2+. Single cell RT-PCR and immunohistochemical studies revealed Cav3.1 (voltage-gated Ca2+ channel) as the main channel subunit detected in PVN-LTS neurons. In conclusion, these data indicate that Cav3.1 is the major subtype of T-type Ca2+ channel subunit that mediates T-type Ca2+ dependent LTS in PVN neurons.
ISSN
0306-4522
Language
English
URI
http://hdl.handle.net/10371/8300
DOI
https://doi.org/10.1016/j.neuroscience.2008.06.055
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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