SHERP

Preventive and therapeutic effects of gene therapy using silica nanoparticles–binding of GM-CSF gene on white blood cell production in dogs with leukopenia

Cited 0 time in webofscience Cited 0 time in scopus
Authors
Choi, Eun Wha; Koo, Hye Cheong; Shin, Il Seob; Chae, Young Jin; Lee, Jong Hwa; Han, Sei Myoung; Lee, Seung Jun; Bhang, Dong Ha; Park, Yong Ho; Lee, Chang Woo; Youn, Hwa Young
Issue Date
2008-06-11
Publisher
Elsevier
Citation
Exp Hematol. 2008;36:1091-1097
Abstract
Objective
Our previous study has shown that granulocyte-macrophage colony-stimulating factor (GM-CSF) gene/silica nanoparticles have a leukocytosis effect in normal dogs. Therefore, this study was conducted to determine whether treatment of canine GM-CSF gene/silica nanoparticles has preventive or therapeutic effects in dogs with leukopenia.

Materials and Methods
To induce leukopenia, vinblastine was administered intravenously at a dose of 2 mg/m2 of body surface area on day 0. Then 7.5 μg GM-CSF/nanoparticles (1:100, w/w) were administered intravenously to each of four dogs in the prevention group on day 2 and an equivalent amount of GM-CSF/nanoparticles was administered to the post-nadir group on day 4 (other groups were administered phosphate-buffered saline intravenously).

Results
Therapeutic GM-CSF gene was expressed in peripheral blood mononuclear cells for 10 days and both the prevention and post-nadir groups showed significant increases in white blood cell counts when compared with the control group, as confirmed by complete blood count, differential count, and flow cytometry.

Conclusions
GM-CSF/nanoparticles can be useful for correction of acute leukopenia, such as chemotherapy-induced myelosuppression, without developing neutralizing antibodies.
ISSN
0301-472X
Language
English
URI
http://hdl.handle.net/10371/8305
DOI
https://doi.org/10.1016/j.exphem.2008.04.002
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse