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Species and Sex Identification of the Korean Goral (Nemorhaedus caudatus) by Molecular Analysis of Non-invasive Samples

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dc.contributor.authorKim, Baek Jun-
dc.contributor.authorLee, Yun-Sun-
dc.contributor.authorAn, Jung-hwa-
dc.contributor.authorPark, Han-Chan-
dc.contributor.authorOkumura, Hideo-
dc.contributor.authorLee, Hang-
dc.contributor.authorMin, Mi-Sook-
dc.date.accessioned2009-08-31T23:49:36Z-
dc.date.available2009-08-31T23:49:36Z-
dc.date.issued2008-07-05-
dc.identifier.citationMol. Cells 26, 314-318en
dc.identifier.issn1016-8478 (print)-
dc.identifier.issn0219-1032 (online)-
dc.identifier.urihttp://hdl.handle.net/10371/8308-
dc.identifier.urihttp://molcells.inforang.com/about/journal_view.html?uid=77-
dc.description.abstractKorean long-tailed goral (Nemorhaedus caudatus) is one of the most endangered species in South Korea. However, detailed species distribution and sex ratio data on the elusive goral are still lacking due to difficulty of identification of the species and sex in the field. The primary aim of this study was to develop an economical PCR-RFLP method to identify species using invasive or non-invasive samples from five Korean ungulates: goral (N. caudatus), roe deer (Capreolus pygargus), feral goat (Capra hircus), water deer (Hydropotes inermis) and musk deer (Moschus moschiferus). The secondary aim was to find more efficient molecular sexing techniques that may be applied to invasive or non-invasive samples of ungulate species. We successfully utilized PCR-RFLP of partial mitochondrial cytochrome b gene (376 bp) for species identification, and sex-specific amplification of ZFX/Y and AMELX/Y genes for sexing. Three species (goral, goat and water deer) showed distinctive band patterns by using three restriction enzymes (XbaI, StuI or SspI). Three different sexing primer sets (LGL331/335 for ZFX/Y gene; SE47/48 or SE47/53 for AMELX/Y gene) produced sex-specific band patterns in goral, goat and roe deer. Our results suggest that the molecular analyses of non-invasive samples might provide us with potential tools for the further genetic and ecological study of Korean goral and related species.en
dc.description.sponsorshipThis study was partially supported by the Research Institute of
Veterinary Science and the Brain Korea 21 Program for Veterinary
Science, Seoul National University. We thank C.S. Jung
and G.L. Park for assisting us in collecting fecal samples of
Korean gorals. We also thank K. Lasater for careful review of
the manuscript.
en
dc.language.isoenen
dc.publisher한국분자·세포생물학회 = Korean Society of Molecular and Cellular Biologyen
dc.subjectamelogeninen
dc.subjectKorean long-tailed goralen
dc.subjectmitochondrial cytochrome ben
dc.subjectPCR-RFLPen
dc.subjectsex identificationen
dc.subjectspecies identificationen
dc.titleSpecies and Sex Identification of the Korean Goral (Nemorhaedus caudatus) by Molecular Analysis of Non-invasive Samplesen
dc.typeArticleen
dc.contributor.AlternativeAuthor김백준-
dc.contributor.AlternativeAuthor이윤선-
dc.contributor.AlternativeAuthor안정화-
dc.contributor.AlternativeAuthor박한찬-
dc.contributor.AlternativeAuthor이항-
dc.contributor.AlternativeAuthor민미숙-
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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