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Screening of cell-penetrating peptides using mRNA display

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Lee, Jae-Hun; Song, Hyun Seok; Lee, Sun-Gu; Park, Tae Hyun; Kim, Byung-Gee
Issue Date
2012-03
Publisher
WILEY-VCH Verlag GmbH & Co. KGaA
Citation
Biotechnology Journal Vol.7 No.3, pp. 387-396
Keywords
의약학Cell-penetrating peptidemRNA displayPeptide screening
Abstract
Cell-penetrating peptides (CPPs) are attractive vectors for in vivo and in vitro cellular uptake. Their use is, however, limited by insufficient understanding of their preference for a target cell. Here, a new CPP screening method is presented that uses mRNA display. After incubating the target cell lines, such as human embryonic kidney 293 (HEK 293) and HeLa cells, with an mRNA display library for 3 h at 37 degrees C, the CPP-mRNA nucleotide conjugates were harvested. These were amplified with PCR and subsequently sequenced. The screened CPPs for each cell line were identified after four rounds of selection. Among them, two peptides, MAMPGEPRRANVMAHKLEPASLQLR NSCA (CPPK) and MAPQRDTVGGRTTPPSWGPAKAQLRNSCA (CPPL) were selected, and the FITC-labeled peptides were evaluated for their ability to penetrate cells. The screened CPPs were superior to polyarginine (R-11), which is widely used as a standard peptide and shows good cell penetration efficiency. Our method can be applied to other target cells for which CPPs have not yet been elucidated.
ISSN
1860-6768(Print)
1860-7314(Online)
Language
English
URI
http://hdl.handle.net/10371/83136
DOI
https://doi.org/10.1002/biot.201100220
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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