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Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA

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Authors
Lee, Hyun Kyoung; Lee, Byoung Hee; Dutta, Noton Kumar; Seok, Seung Hyeok; Baek, Min Won; Lee, Hui Young; Kim, Dong Jae; Na, Yi Rang; Noh, Kyoung Jin; Park, Sung Hoon; Kariwa, Hiroaki; Nakauchi, Mina; Quynh Mai, Le; Heo, Suk-Jin; Park, Jae Hak
Issue Date
2008
Publisher
한국미생물생명공학회 = The Korean Society for Applied Microbiology
Citation
J. Microbiol. Biotechnol. 18:1717-1721
Keywords
ELISASARStruncated recombinant nucleocapsid proteins
Abstract
Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.
ISSN
1017-7825
Language
English
URI
http://hdl.handle.net/10371/8315
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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