Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA

Cited 0 time in webofscience Cited 0 time in scopus
Lee, Hyun Kyoung; Lee, Byoung Hee; Dutta, Noton Kumar; Seok, Seung Hyeok; Baek, Min Won; Lee, Hui Young; Kim, Dong Jae; Na, Yi Rang; Noh, Kyoung Jin; Park, Sung Hoon; Kariwa, Hiroaki; Nakauchi, Mina; Quynh Mai, Le; Heo, Suk-Jin; Park, Jae Hak
Issue Date
한국미생물생명공학회 = The Korean Society for Applied Microbiology
J. Microbiol. Biotechnol. 18:1717-1721
ELISASARStruncated recombinant nucleocapsid proteins
Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.
Files in This Item:
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.