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Characterization of a T7-Like Lytic Bacteriophage (SG-JL2) of Salmonella enterica Serovar Gallinarum Biovar Gallinarum

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Authors
Kwon, Hyuk-Joon; Cho, Sun-Hee; Kim, Tae-Eun; Won, Yong-Jin; Jeong, Jihye; Park, Se Chang; Kim, Jae-Hong; Yoo, Han Sang; Park, Yong Ho; Kim, Sun-Joong
Issue Date
2008
Publisher
American Society for Microbiology
Citation
Appl. Environ. Microbiol. 74:6970-6979
Abstract
SG-JL2 is a newly discovered lytic bacteriophage infecting Salmonella enterica serovar Gallinarum
biovar Gallinarum but is nonlytic to a rough vaccine strain of serovar Gallinarum biovar Gallinarum
(SG-9R), S. enterica serovar Enteritidis, S. enterica serovar Typhimurium, and S. enterica serovar Gallinarum
biovar Pullorum. The SG-JL2 genome is 38,815 bp in length (GC content, 50.9%; 230-bp-long
direct terminal repeats), and 55 putative genes may be transcribed from the same strand. Functions were
assigned to 30 genes based on high amino acid similarity to known proteins. Most of the expected proteins
except tail fiber (31.9%) and the overall organization of the genomes were similar to those of yersiniophage
YeO3-12. SG-JL2 could be classified as a new T7-like virus and represents the first serovar Gallinarum
biovar Gallinarum phage genome to be sequenced. On the basis of intraspecific ratios of nonsynonymous
to synonymous nucleotide changes (Pi[a]/Pi[s]), gene 2 encoding the host RNA polymerase inhibitor
displayed Darwinian positive selection. Pretreatment of chickens with SG-JL2 before intratracheal
challenge with wild-type serovar Gallinarum biovar Gallinarum protected most birds from fowl typhoid.
Therefore, SG-JL2 may be useful for the differentiation of serovar Gallinarum biovar Gallinarum from
other Salmonella serotypes, prophylactic application in fowl typhoid control, and understanding of the
vertical evolution of T7-like viruses.
ISSN
0099-2240
Language
English
URI
http://hdl.handle.net/10371/8322
DOI
https://doi.org/10.1128/AEM.01088-08
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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