Publications
Detailed Information
Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seung Tae | - |
dc.contributor.author | Choi, Mun Hwan | - |
dc.contributor.author | Lee, Eun Ju | - |
dc.contributor.author | Gong, Seung Pyo | - |
dc.contributor.author | Jang, Mi | - |
dc.contributor.author | Park, Sang Hyun | - |
dc.contributor.author | Jee, Hyang | - |
dc.contributor.author | Kim, Dae-Yong | - |
dc.contributor.author | Han, Jae Yong | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.date.accessioned | 2009-09-01 | - |
dc.date.available | 2009-09-01 | - |
dc.date.issued | 2007-04-19 | - |
dc.identifier.citation | Fertil Steril 2008;90:1910-1920 | en |
dc.identifier.issn | 0015-0282 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8324 | - |
dc.description.abstract | Objective: To evaluate whether autologous embryonic stem cells can be established without generating clone embryos.
Design: Prospective model study. Setting: Gamete and stem cell biotechnology laboratory in Seoul National University, Seoul, Korea. Animal(s): F1 hybrid B6D2F1 mice. Intervention(s): Preantral follicles were cultured, and oocytes matured in the follicles were parthenogenetically activated. Main Outcome Measure(s): Preimplantation development and stem cell characterization. Result(s): More intrafollicular oocytes that were retrieved from secondary follicles matured and developed into blastocysts after parthenogenesis than those that were retrieved from primary follicles. Of those 35 blastocysts derived from 193 parthenotes, one line of colony-forming cells was established from the culturing of early secondary follicles. The established cells were positive for embryonic stem cell-specific markers and had normal diploid karyotype and telomerase activity. They differentiated into embryoid bodies in vitro and teratomas in vivo. Inducible differentiation of the established cells into neuronal lineage cells also was possible. Conclusion(s): Autologous embryonic stem cells can be established by preantral follicle culture and oocyte parthenogenesis. A combined technique of follicle culture and oocyte parthenogenesis that does not use developmentally competent oocytes has the potential to replace somatic cell nuclear transfer for autologous cell therapy. | en |
dc.description.sponsorship | Supported by a grant from the Stem Cell Research Center of the 21st Century
Frontier Research Program, funded by the Ministry of Science and Technology (Gwacheon, Korea; SC-2170). Also supported by a graduate fellowship from the Korean Ministry of Education, Brain Korea 21 project (Seoul, Korea). | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Preantral follicle | en |
dc.subject | intrafollicular oocyte | en |
dc.subject | in vitro-culture | en |
dc.subject | parthenogenesis | en |
dc.subject | blastocyst | en |
dc.subject | autologous stem cell | en |
dc.subject | characterization | en |
dc.title | Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이승태 | - |
dc.contributor.AlternativeAuthor | 최문환 | - |
dc.contributor.AlternativeAuthor | 이은주 | - |
dc.contributor.AlternativeAuthor | 공승표 | - |
dc.contributor.AlternativeAuthor | 장미 | - |
dc.contributor.AlternativeAuthor | 박상현 | - |
dc.contributor.AlternativeAuthor | 지향 | - |
dc.contributor.AlternativeAuthor | 김대용 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.identifier.doi | 10.1016/j.fertnstert.2007.01.099 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.