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Human Taste Receptor-Functionalized Field Effect Transistor as a Human-Like Nanobioelectronic Tongue

Cited 61 time in Web of Science Cited 69 time in Scopus
Authors
Song, Hyun Seok; Kwon, Oh Seok; Lee, Sang Hun; Park, Seon Joo; Kim, Un-Kyung; Jang, Jyongsik; Park, Tai Hyun
Issue Date
2012-11-26
Publisher
American Chemical Society
Citation
Nano Letters Vol.13 No.1, pp. 172-178
Keywords
복합학Human taste receptorconducting polymernanotubefield-effect transistornanobioelectronic tongueantithyroid toxin
Abstract
In this study, we developed a human taste receptor protein, hTAS2R38-functionalized carboxylated polypyrrole nanotube (CPNT)-field effect transistor (PET) as a nanobioelectronic tongue (nbe-tongue) that displayed human-like performance with high sensitivity and selectivity. Taster type (PAV) and nontaster type (AVI) hTAS2R38s were expressed in Escherichia coli (E. coli) at a high level and immobilized on a CPNT-FET sensor platform. Among the various tastants examined, PAV-CPNT-FET exclusively responded to target bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP), with high sensitivity at concentrations as low as 1 fM. However, no significant changes were observed in the AVI-CPNT-FET in response to the target bitter tastants. This nbe-tongue exhibited different bitter-taste perception of compounds containing thiourea (N-C=S) moieties such as PTC, PROP, and antithyroid toxin in vegetables, which corresponded to the haplotype of hTAS2R38 immobilized on CPNTs. This correlation with the type of receptor is very similar to the human taste system. Thus, the artificial taste sensor developed in this study allowed for the efficient detection of target tastants in mixture and real food sample with a human-like performance and high sensitivity. Furthermore, our nbe-tongue could be utilized as a substitute for cell-based assays and to better understand the mechanisms of human taste.
ISSN
1530-6984 (print)
1530-6992 (web)
Language
English
URI
http://hdl.handle.net/10371/83299
DOI
https://doi.org/10.1021/nl3038147
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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