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Human Taste Receptor-Functionalized Field Effect Transistor as a Human-Like Nanobioelectronic Tongue

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dc.contributor.authorSong, Hyun Seok-
dc.contributor.authorKwon, Oh Seok-
dc.contributor.authorLee, Sang Hun-
dc.contributor.authorPark, Seon Joo-
dc.contributor.authorKim, Un-Kyung-
dc.contributor.authorJang, Jyongsik-
dc.contributor.authorPark, Tai Hyun-
dc.creator박태현-
dc.date.accessioned2013-08-14T00:50:58Z-
dc.date.available2013-08-14T00:50:58Z-
dc.date.issued2012-11-26-
dc.identifier.citationNano Letters Vol.13 No.1, pp. 172-178-
dc.identifier.issn1530-6984 (print)-
dc.identifier.issn1530-6992 (web)-
dc.identifier.urihttp://hdl.handle.net/10371/83299-
dc.description.abstractIn this study, we developed a human taste receptor protein, hTAS2R38-functionalized carboxylated polypyrrole nanotube (CPNT)-field effect transistor (PET) as a nanobioelectronic tongue (nbe-tongue) that displayed human-like performance with high sensitivity and selectivity. Taster type (PAV) and nontaster type (AVI) hTAS2R38s were expressed in Escherichia coli (E. coli) at a high level and immobilized on a CPNT-FET sensor platform. Among the various tastants examined, PAV-CPNT-FET exclusively responded to target bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP), with high sensitivity at concentrations as low as 1 fM. However, no significant changes were observed in the AVI-CPNT-FET in response to the target bitter tastants. This nbe-tongue exhibited different bitter-taste perception of compounds containing thiourea (N-C=S) moieties such as PTC, PROP, and antithyroid toxin in vegetables, which corresponded to the haplotype of hTAS2R38 immobilized on CPNTs. This correlation with the type of receptor is very similar to the human taste system. Thus, the artificial taste sensor developed in this study allowed for the efficient detection of target tastants in mixture and real food sample with a human-like performance and high sensitivity. Furthermore, our nbe-tongue could be utilized as a substitute for cell-based assays and to better understand the mechanisms of human taste.en
dc.description.sponsorshipThis work was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) (Grant Nos. 2012-0000144, 2012K001365, 2012-0006564). This research was supported by the WCU (World Class University) program through the NRF, funded by the MEST (R31-10013), and funded by an NRF grant through the Korean government (MEST) (No. 2011-0017125).-
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.subject복합학en
dc.subjectHuman taste receptor-
dc.subjectconducting polymer-
dc.subjectnanotube-
dc.subjectfield-effect transistor-
dc.subjectnanobioelectronic tongue-
dc.subjectantithyroid toxin-
dc.titleHuman Taste Receptor-Functionalized Field Effect Transistor as a Human-Like Nanobioelectronic Tongueen
dc.typeArticle-
dc.author.alternative송현석-
dc.author.alternative권오석-
dc.author.alternative이상헌-
dc.author.alternative박선주-
dc.author.alternative김은경-
dc.author.alternative장정식-
dc.author.alternative박태현-
dc.identifier.doi10.1021/nl3038147-
dc.citation.journaltitleNano Letters-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000002410/4-
dc.description.srndSEQ:4-
dc.description.srndPERF_CD:SNU2013-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000002410-
dc.description.srndADJUST_YN:Y-
dc.description.srndEMP_ID:A002014-
dc.description.srndDEPT_CD:458-
dc.description.srndCITE_RATE:13.198-
dc.description.srndFILENAME:human taste receptor-functionalized.pdf-
dc.description.srndDEPT_NM:화학생물공학부-
dc.description.srndEMAIL:thpark@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.rimsid39519-
dc.identifier.srnd2013-01/102/0000002410/4-
dc.type.rimsART-
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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