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Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells

DC Field Value Language
dc.contributor.authorSeo, Min-Soo-
dc.contributor.authorPark, Sang-Bum-
dc.contributor.authorKim, Hyung-Sik-
dc.contributor.authorKang, Jun-gu-
dc.contributor.authorChae, Joon-Seok-
dc.contributor.authorKang, Kyung-Sun-
dc.creator채준석-
dc.date.accessioned2013-09-06T01:44:44Z-
dc.date.available2013-09-06T01:44:44Z-
dc.date.issued2013-06-
dc.identifier.citationJournal of Veterinary Science Vol.14 No.2, pp. 151-159-
dc.identifier.issn1229-845X (print)-
dc.identifier.issn1976-555X (online)-
dc.identifier.urihttps://hdl.handle.net/10371/83362-
dc.description.abstractRecent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs) that were cultured and maintained in low glucose Dulbeccos modified Eagles medium. The proliferation of eAM-MSCs was measured based on the cumulative population doubling level (CPDL). Immunophenotyping of eAM-MSCs by flow cytometry showed that the major population was of mesenchymal origin. To confirm differentiation potential, a multi-lineage differentiation assay was conducted. We found that under appropriate conditions, eAM-MSCs are capable of multi-lineage differentiation. Our results indicated that eAM-MSCs may be a good source of stem cells, making them potentially useful for veterinary regenerative medicine and cell-based therapy.-
dc.description.sponsorshipThis work was supported by a National Research
Foundation of Korea (NRF) grant funded by the Korean
government (MEST, 2010-0020265).
-
dc.language.isoenen
dc.publisherThe Korean Society of Veterinary Scienceen
dc.publisher대한수의학회-
dc.subject의약학en
dc.subjectamnion-
dc.subjectamniotic membrane-
dc.subjectequine-
dc.subjectisolation-
dc.subjectmesenchymal stem cells-
dc.titleIsolation and characterization of equine amniotic membrane-derived mesenchymal stem cellsen
dc.typeArticle-
dc.author.alternative서민수-
dc.author.alternative박상범-
dc.author.alternative김형식-
dc.author.alternative강준구-
dc.author.alternative채준석-
dc.author.alternative강경선-
dc.identifier.doi10.4142/jvs.2013.14.2.151-
dc.citation.journaltitleJournal of Veterinary Science-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000051105/4-
dc.description.srndSEQ:4-
dc.description.srndPERF_CD:SNU2013-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000051105-
dc.description.srndADJUST_YN:N-
dc.description.srndEMP_ID:A077262-
dc.description.srndDEPT_CD:551-
dc.description.srndCITE_RATE:1.161-
dc.description.srndFILENAME:2013 jvs 14(2)151-159-equine stem cell.pdf-
dc.description.srndDEPT_NM:수의학과-
dc.description.srndEMAIL:jschae@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndCONFIRM:Y-
dc.identifier.srnd2013-01/102/0000051105/4-
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