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Chemical modification of chitosan as a gene carrier in vitro and in vivo

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Authors
Kim, Tae-Hee; Jiang, Hu-Lin; Jere, Dhananjay; Park, In-Kyu; Cho, Myung-Haing; Nah, Jae-Woon; Choi, Yun-Jaie; Akaike, Toshihiro; Cho, Chong-Su
Issue Date
2007-05-21
Publisher
Elsevier
Citation
Prog. Polym. Sci. 2007;32:726-753
Keywords
ChitosanGene deliveryCell specificityTransfection efficiencyChemical modification
Abstract
Currently, the success of gene therapy is mainly limited due to the lack of effective vector systems. Although viral vectors are highly efficient in transfecting cells, undesirable complications limit their therapeutic applications. Chitosan has been investigated as a non-viral vector offering several advantages, such as biocompatibility, biodegradability and low toxicity with high cationic potential. However, the low transfection efficiency and low cell specificity of chitosan as a DNA carrier need to be overcome before undertaking clinical trials. The objective of this review is to summarize the use of chitosan and chitosan derivatives in gene therapy, and particularly the role of several factors for the enhancement of transfection efficiency and cell specificity in vitro, such as the degree of deacetylation and molecular weight of chitosan, pH, serum, charge ratio of chitosan to DNA and cell type on transfection efficiency, chemical modification. The administration of the chitosan derivative formulations in vivo is also included, and, the role of chitosan as a carrier of controlled release of DNA and small interfering RNA is described.
ISSN
0079-6700
Language
English
URI
http://hdl.handle.net/10371/8337
DOI
https://doi.org/10.1016/j.progpolymsci.2007.05.001
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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