SHERP

A Degradable Hyperbranched Poly(ester amine) Based on Poloxamer Diacrylate and Polyethylenimine as a Gene Carrier

Cited 0 time in webofscience Cited 0 time in scopus
Authors
Kim, Tae Hee; Cook, Seung Eun; Arote, Rohidas B.; Cho, Myung-Haing; Nah, Jae Woon; Choi, Yun Jaie; Cho, Chong Su
Issue Date
2007-04-25
Publisher
John Wiley & Sons
Citation
Macromol. Biosci. 2007, 7, 611
Keywords
biodegradable polymersgene deliveryhyperbranched poly(ester amine)poloxamerpolyethylenimine
Abstract
Polyethylenimine (PEI) is a well-known cationic polymer which has high transfection efficiency due to its buffering effect. However, nondegradability, cytotoxicity, aggregation, and short-circulation time in vivo still need to be overcome for a successful gene delivery. Degradable, hyperbranched poly(ester amine)s (PEAs) based on poloxamer diacrylate and low molecular weight branched PEI, were successfully synthesized and evaluated as a nonviral gene carrier. The PEAs were obtained in significant yields through Michael type addition reaction of diacrylate monomers and low molecular weight branched PEI. Analysis of degradation products by the reduction in molecular weight demonstrated that PEAs degrade in a controlled fashion. The PEA showed good DNA binding ability and the sizes of complexes under physiological condition were below 150 nm, implicating its potential for intracellular delivery. It showed lower cytotoxicity in three different cell lines (A549, 293T, and HepG2) compared with PEI 25K. PEAs showed much higher transfection efficiencies in three cell lines compared with PEI 25K and PEI 1.8K, and revealed little serum dependency in A549 cell line when the content of poloxamer in the PEA was increased up to 30%.
ISSN
1616-5187 (print)
1616-5195 (online)
Language
English
URI
http://hdl.handle.net/10371/8343
DOI
https://doi.org/10.1002/mabi.200600245
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse