효소표식항체법에의한 Human Chorionic Gonadotropin의 흰쥐 조직내 위치검정에 관한 연구

Abstract

Forty eight male rats weighing 140--170gm were injected intravenously with 1 to 30 mg of human chorionic gonadotropin. The localization of this hormone within 5 minutes to 24 hours after injection was carried out immunohistochernically using the specific rabbit anti-human chorionic gonadotropin and sheep anti-rabbit gamma globulin labeled with horseradish peroxidase followed by enzyme staining and then was examined under the ordinary light microscope. The most frequent and intense reaction sites were the cells of Leydig of the testis, epithelial cells of ductus epididymis, and ductus deferens. Some localization of hormone was also observed in the tunica albuginea of the testis, macrophages of the mesenteric lymph node, and Kupffer cells of the liver. No positive reactions were observed in the thyroid gland and the kidney. In the pituitary gland, endogenous luteinizing hormone which is known as having specific cross reaction with the anti-human chorionic gonadotropin was localized intensely both in the experimental and control group of rats. Positive reaction was appeared weakly 15 minutes after injection of hormone and was reached to maximum intensity 60 minutes after injection, therafter intensities were reduced gradually. We failed to localize HCG in the rats which were injected 1 mg and 5 mg of hormone respectively. Contol group of rats did not reveal any positive reaction site except the luteinizing hormone granules of the pituitary glands.