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Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

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Authors
Kwon, Keon Woo; Choi, Sung Sik; Lee, Sang Ho; Kim, Byungkyu; Lee, Se Na; Park, Min Cheol; Kim, Pilnam; Hwang, Se Yon; Suh, Kahp Y.
Issue Date
2007
Publisher
Royal Society of Chemistry
Citation
Lab Chip, 2007, 7, 1461-1468
Keywords
PLATE FLOW CHAMBERSHEAR-FLOWMODELCARCINOMASURFACECOLLECTIONEXPRESSIONCHANNELPROBES
Abstract
A label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e. g., one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 mu l min(-1). The fraction of MCF7 cells was increased from 0.36 +/- 0.04 to 0.83 +/- 0.04 under these optimized conditions.
ISSN
1473-0197
Language
English
URI
http://hdl.handle.net/10371/8378
DOI
https://doi.org/10.1039/b710054j
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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