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Label-free, microfluidic separation and enrichment of human breast cancer cells by adhesion difference

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dc.contributor.authorKwon, Keon Woo-
dc.contributor.authorChoi, Sung Sik-
dc.contributor.authorLee, Sang Ho-
dc.contributor.authorKim, Byungkyu-
dc.contributor.authorLee, Se Na-
dc.contributor.authorPark, Min Cheol-
dc.contributor.authorKim, Pilnam-
dc.contributor.authorHwang, Se Yon-
dc.contributor.authorSuh, Kahp Y.-
dc.date.accessioned2009-09-01T03:43:56Z-
dc.date.available2009-09-01T03:43:56Z-
dc.date.issued2007-
dc.identifier.citationLab Chip, 2007, 7, 1461-1468en
dc.identifier.issn1473-0197-
dc.identifier.urihttp://hdl.handle.net/10371/8378-
dc.description.abstractA label-free microfluidic method for separation and enrichment of human breast cancer cells is presented using cell adhesion as a physical marker. To maximize the adhesion difference between normal epithelial and cancer cells, flat or nanostructured polymer surfaces (400 nm pillars, 400 nm perpendicular, or 400 nm parallel lines) were constructed on the bottom of polydimethylsiloxane (PDMS) microfluidic channels in a parallel fashion using a UV-assisted capillary moulding technique. The adhesion of human breast epithelial cells (MCF10A) and cancer cells (MCF7) on each channel was independently measured based on detachment assays where the adherent cells were counted with increasing flow rate after a pre-culture for a period of time (e. g., one, two, and four hours). It was found that MCF10A cells showed higher adhesion than MCF7 cells regardless of culture time and surface nanotopography at all flow rates, resulting in label-free separation and enrichment of cancer cells. For the cell types used in our study, an optimum separation was found for 2 hours pre-culture on the 400 nm perpendicular line pattern followed by flow-induced detachment at a flow rate of 200 mu l min(-1). The fraction of MCF7 cells was increased from 0.36 +/- 0.04 to 0.83 +/- 0.04 under these optimized conditions.en
dc.description.sponsorshipThis work was supported by the Ministry of Science and Technology through Bio Tool R&D Project for Cell Research and in part by the Micro Thermal System Research Center of
Seoul National University.
en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.subjectPLATE FLOW CHAMBERen
dc.subjectSHEAR-FLOWen
dc.subjectMODELen
dc.subjectCARCINOMAen
dc.subjectSURFACEen
dc.subjectCOLLECTIONen
dc.subjectEXPRESSIONen
dc.subjectCHANNELen
dc.subjectPROBESen
dc.titleLabel-free, microfluidic separation and enrichment of human breast cancer cells by adhesion differenceen
dc.typeArticleen
dc.contributor.AlternativeAuthor권건우-
dc.contributor.AlternativeAuthor최성식-
dc.contributor.AlternativeAuthor이상호-
dc.contributor.AlternativeAuthor김병규-
dc.contributor.AlternativeAuthor이세나-
dc.contributor.AlternativeAuthor박민철-
dc.contributor.AlternativeAuthor김필남-
dc.contributor.AlternativeAuthor황세연-
dc.contributor.AlternativeAuthor서갑양-
dc.identifier.doi10.1039/b710054j-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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