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Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts

Cited 9 time in Web of Science Cited 11 time in Scopus
Authors

Choresca Jr., Casiano H.; Koo, Ok Jae; Oh, Hyun Ju; Hong, So Gun; Gomez, Dennis K.; Kim, Ji Hyung; Lee, Byeong Chun; Park, Se Chang

Issue Date
2008-10-17
Publisher
Elsevier
Citation
Cell Biol Int 2009;33:65-70
Keywords
Cell cycle synchronizationG0/G1 phaseGoldfishSerum starvationCulture to confluenceRoscovitine
Abstract
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 μM) induce cell cycle arrest at the G0/G1 phase.
ISSN
1065-6995
Language
English
URI
https://hdl.handle.net/10371/8605
DOI
https://doi.org/10.1016/j.cellbi.2008.09.015
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