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Real-time monitoring of S-adenosyl-L-homocysteine hydrolase using a chemodosimetric fluorescence "turn-on" sensor

Cited 4 time in Web of Science Cited 4 time in Scopus
Authors

Lee, Kyung-Sik; Lee, Seung Hwan; Oh, Jinrok; Shin, Ik-Soo; Park, Tai Hyun; Hong, Jong-In

Issue Date
2013-08
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, Vol.185, pp.663-668
Abstract
Although various methods have been used to measure the activity of S-adenosyl-L-homocysteine hydrolase (SAHase), most of them are rather complex, expensive, and not suitable for routine analysis. Herein, we describe simple fluorescence monitoring for SAHase activity using a synthetic probe that undergoes chemical binding selectively with L-homocysteine (Hcy), an enzyme metabolite from SAHase catalysis. The ring formation between the probe and Hcy during the enzyme catalysis is quantified by fluorescent emission from the binding adduct, which provides facile, real-time monitoring for the SAHase activity. The chemodosimetric reaction between the probe and Hcy is similar to 100-fold faster than that for the enzyme catalysis so that it is successfully applicable in SAHase assay. (C) 2013 Elsevier B.V. All rights reserved.
ISSN
0925-4005
Language
English
URI
https://hdl.handle.net/10371/90921
DOI
https://doi.org/10.1016/j.snb.2013.05.045
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