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Real-time monitoring of S-adenosyl-L-homocysteine hydrolase using a chemodosimetric fluorescence "turn-on" sensor

Cited 4 time in Web of Science Cited 4 time in Scopus
Authors
Lee, Kyung-Sik; Lee, Seung Hwan; Oh, Jinrok; Shin, Ik-Soo; Park, Tai Hyun; Hong, Jong-In
Issue Date
2013-08
Publisher
Elsevier B.V.
Citation
Sensors and Actuators B: Chemical Vol.185, pp. 663-668
Keywords
복합학S-adenosyl-l-homocysteine hydrolaseChemodosimeterCoumarinFluorescent assayl-Homocysteine
Abstract
Although various methods have been used to measure the activity of S-adenosyl-L-homocysteine hydrolase (SAHase), most of them are rather complex, expensive, and not suitable for routine analysis. Herein, we describe simple fluorescence monitoring for SAHase activity using a synthetic probe that undergoes chemical binding selectively with L-homocysteine (Hcy), an enzyme metabolite from SAHase catalysis. The ring formation between the probe and Hcy during the enzyme catalysis is quantified by fluorescent emission from the binding adduct, which provides facile, real-time monitoring for the SAHase activity. The chemodosimetric reaction between the probe and Hcy is similar to 100-fold faster than that for the enzyme catalysis so that it is successfully applicable in SAHase assay.
ISSN
0925-4005
Language
English
URI
http://hdl.handle.net/10371/90921
DOI
https://doi.org/10.1016/j.snb.2013.05.045
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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