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Effects of porous beta-tricalcium phosphate-based ceramics used as an E-coli-derived rhBMP-2 carrier for bone regeneration

Cited 17 time in Web of Science Cited 24 time in Scopus
Authors
Lee, Jae Hyup; Ryu, Mi Young; Baek, Hae-Ri; Lee, Kyung Mee; Seo, Jun-Hyuk; Lee, Hyun-Kyung; Ryu, Hyun-Seung
Issue Date
2013-09
Publisher
Springer US
Citation
Journal of Materials Science: Materials in Medicine Vol.24 No.9, pp. 2117-2127
Keywords
복합학
Abstract
Recombinant human bone morphogenetic protein-2 (rhBMP-2) requires carriers for clinical effectiveness. In this study, whether porous beta-tricalcium phosphate (beta-TCP)-based ceramics are ideal carriers for rhBMP-2 was investigated. Hydroxyapatite (HA), beta-TCP, TCP/HA (80 %/20 %), HA with rhBMP-2, TCP with rhBMP-2, and TCP/HA (80 %/20 %) with rhBMP-2 were manufactured by a sponge method with a pore size of 300 mu m or more and macro-porosity of 83 %. The alkaline phosphatase (ALP) activity and ALP expression of the cells with 100 % beta-TCP granules were more increased than the those of cells with 100 % HA and TCP/HA (80 %/20 %) at the baseline or when treated with 15 ng/ml of rhBMP-2. In an SD rat calvarial defect model, new bone formation was evidently shown in the TCP 100 %-rhBMP-2 and TCP/HA (80 %/20 %)-rhBMP-2 groups, showing that the most affected area was filled with newly-formed bone, that the percent bone volume and trabecular number were larger when compared to the groups without rhBMP-2 treatment at both 4 and 8 weeks after surgery using micro-CT and histology. Porous TCP-based ceramic granules enhanced the osteoblastic differentiation in the hMSC system when treated with 15 ng/ml of rhBMP-2 and accelerated bone-healing by trabecular number in a rat calvarial defect model. Thus, in this study it was proposed that TCP-based ceramics might be useful carriers of rhBMP-2.
ISSN
0957-4530 (print)
1573-4838 (online)
Language
English
URI
http://hdl.handle.net/10371/90954
DOI
https://doi.org/10.1007/s10856-013-4967-5
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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