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Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles

Cited 28 time in Web of Science Cited 29 time in Scopus
Authors
Lee, Hong Jai; Park, Hee Ho; Kim, Jeong Ah; Park, Ju Hyun; Ryu, Jina; Choi, Jeongseon; Lee, Jongmin; Rhee, Won Jong; Park, Tai Hyun
Issue Date
2014-02
Publisher
Elsevier Ltd
Citation
Biomaterials Vol.35 No.5, pp. 1696-1704
Keywords
복합학ProteinProtein nanoparticleDrug deliveryDrug releaseEnzyme stability
Abstract
Nanoparticles have been widely used for delivering various chemical and biomolecular drugs, such as anti-cancer drugs and therapeutic proteins. Among nanoparticles, protein nanoparticles have advantages of non-cytotoxicity and biodegradability. In this study, a recombinant 30Kc19 protein was applied to human serum albumin (HSA) nanoparticles to enhance cellular uptake and stability of a nanoparticle cargo enzyme. The 30Kc19 protein, which originates from silkworm, has cell-penetrating and enzyme-stabilizing abilities. Therefore, 30Kc19–HSA nanoparticles were expected to enhance cellular uptake and stability of an enzyme loaded on the nanoparticles. Here, nanoparticles loaded with β-galactosidase were prepared using the desolvation method. The 30Kc19–HSA nanoparticles were uniformly spherical in shape, dispersed evenly in phosphate buffered saline and cell culture media, and released β-galactosidase in a sustained manner. The 30Kc19–HSA nanoparticles had negligible toxicity to animal cells and exhibited enhanced cellular uptake and intracellular stability of β-galactosidase in HeLa and HEK293 cells when compared with those of HSA nanoparticles. These results suggest that 30Kc19–HSA protein nanoparticles could be used as a versatile tool for drug delivery to various cells.
ISSN
0142-9612
Language
English
URI
http://hdl.handle.net/10371/91297
DOI
https://doi.org/10.1016/j.biomaterials.2013.11.001
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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