S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 04 No.3 (1963)
포도구균의 알파-용혈소와 그 항체에 관한 연구 알파-용혈소의 생활, 성질 및 항 알파-용혈소치의 임상적의의
Studies on Staphylococcal α-Hemolysin & Its Antibody
- Issue Date
- 서울대학교 의과대학
- Seoul J Med 1963;4(2):39-56
- National Medical Center, Seoul, Korea
The confirmation of deep-seated staphylococcal
infections is one of the most difficult diagnostic
problem. As with Anti-Streptolysin “ 0 " Titer, the same
measure may be implied to the staphylococcal cases.
Since 1935, many ~articles have' appeared on this
problem but none of them has presented a critical
value or standard as a diagnostic criterion. This paper
presents more than 150 cases of staphylococcal infections
, both superficial and deep-seated, with particular
consideration for the applicability of anti-staphylolysin titer CASTA) in patient as one of the diagnostic
In order to secure the alpha-hemolysin of staphylococcus,
Wood 46 strain and standard antitoxin of
staphylococcus from the State Serum Institute of
Denmark were used for the production of staphylolysin
and identification. As a preliminary test, factors
influencing alpha-hemolysin production in media were
tested. A high titer alpha-hemolysin was produced
after 5 days culture in brain heart infusion broth in
an atmospheric condition of 8-10 % CO2 tension. The
nature of alpha-hemolysin was tested on various
erythrocytes, on influences by pH changes, and on
heat resistance. Finally, the identity of the alpha-hemolysin
produced was confirmed by a neutralization
test with standard antitoxin.
Anti-staphylolysin tests in vitro were performed on
552 sera, taken from apparently normal individuals,
and on 235 sera from various patients including those
infected by staphylococci. The results are summarized
1. During the production of a potent alpha-hemolysin
in vitro, it seemed essential that the CO2 tension
be kept at 8-10 %.
2. The hemolytic activities of alpha-hemolysin
revealed various sensitivity on animal erythrocytes in
decreasing order as follows: rabbit, swine, cattle,
sheep , and rat. Human, horse or guinea pig erythrocytes
were not hemolyzed.
3. Pseudomonas aeruginosa destroyed the alphahemolysin
very rapidly when the organisms were
cultivated in a culture filtrate containing alpha-hemolysin.
4. A storage test of the fluid alpha-hemolysin at
4a C showed practically no change in titer within one
5. For the application of ASTA, the following
criteria are suggested.
Insignificant or Normal: less than 2 u.
Borderline group or inconclusive: 2-4 u.
Significant or pathologic 4 u. or more.
6. Among spondylitis or osteomyelitis of unknown
etiology, at least 20% were confirmed as being of
staphylococcal origin, when judged by ASTA elevation
or positive culture. The positive cultures in the cases
of deep-seated staphylococcal infections were usually
accompanied by high titers of ASTA. Three cases of
septicemia showed marked elevation of ASTA.
7. In most of the superficial staphylococcal infections
no elevation of ASTA was found , but in some cases
of deep seated infections considerable elevations of
ASTA were found even when the culture tests were