Acute stress가 마우스의 간조직 및 혈청내 RNase와 RNase inhibitor 활성에 미치는 영향

Abstract

Acute stress was induced to the mice by intraperitoneal injection of celite suspension. The activities of RNase and RNase inhibitor were examined on the liver and serum with the time intervals of 4, 8, 12, 16 and 20 hours after induction of acute stress, and adrenal ascorbic acid was also determined to testify the effectiveness of such a stress inducing method. The results obtained are as follows. 1. The activity of hepatic acid RNase was markedly decreased as much as 41%, as compared to control value, 8 hours after inducing acute stress. 2. No change was observed in the activity of hepatic and serum total RNase by acute stress, but the activity of liver alkaline RNase was decreased 31% 12 hours following acute stress and that of serum alkaline RNase was decreased 32% 8 hours after inducing stress. The lowered RNase activities were gradually increased to normal value 20 hours after celi te injection. The acute stress was thus shown to induce a marked decrease in RNase activities of the liver and serum, 3. The hepatic RNase inhibitor was confirmed to be bound to alkaline RNase , but not to acid RNase. 4. The content of adrenal ascorbic acid was diminished gradually from the initiation of acute sress, reaching to minimum level 8 hours after initiating celite induced stress, followed by gradual increase to control leve l.