히스타민으로 유발시킨 피부염증 부위의 RISA 클리어런스 및 수분 이동
RISA Clearance and Water Shift in Skin Inflammatory Region Induced by Histamine Injection

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서울대학교 의과대학
Seoul J Med 1966;7(4):33-41
Movements of RISA across capillary membrane after
intradermal injection of histamine were studied in anesthetized
rabbits. Intravenous injection of 2 ml saline
solution containing 30-100 μC of RISA and 1 % Evans
blue dye was given to the animal. The dye served as
a guide in delineating the area of inflammatory tissues.
In order to ensure uniform distribution of the injected
materials, 10 minutes were allowed to elapse before
saline and logarithmic doses of histamine dihydrochloride
in a volume of 0.05 ml were injected intradermally.
A total of 7 sites were injected on the abdominal skin of each animal. Ten minutes after the intradermal
injections, animal was killed , the skin sites
were excised and blood samples were taken by cardiac
punctures. From the tissue and blood samples , radioactivities
of RISA, concentration of sodium and tissue
water content were analyzed. Calculations were made
on contents of tissue water, Na space as a size of extracellular
fluid space, size of intracellular fluid and
local clearances of RISA.
The following results were obtained.
1. Tissue water content of skin increased as the injected
dose of histamine increased. The dose-response
-curve showed a S-shaped curve. Tissue water content
at the control skin site was 67.8±2. 3%. At 10- 3μg
histamine injected skin site the tissue water content
was only slightly greater than that of the control. As
the injected dose of histamine increased up to a dose of
101μg, skin tissue water content increased steeply_
Thereafter skin tissue water content maintained a maximum
plateau value of 76.0±2. 9% at a dose of 102
μg histamine.
2. Histamine dose-response curves of extracellular
and intracellular fluid were similar to the tissue water
change showing a typical sigmoid curve. The size of
‘ extracellular fluid volume determined as Na-space shoo
wed 41.5±1.0% in the control site and increased to
the maximum plateau value of 44.8±1. 9 % at the
dose of 102μ g histamine. The size of intracellular
fluid volume determined by difference between total tissue
water and Na-space was averaged of 26.3 %in
the control site and increased to the maximum plateau
value of 31. 2 % in the site injected 102μ g of hista·
3. The RISA clearance was averaged O. 036±0. 005
cc/gm at the control site which value represents the
plasma volume of normal skin. Clearances in the
inflammatory sites induced by graded dose of histamine
increased with the same fashion as in the change in
tissue water. Thus, a modest increase at a dose of 10-3
μg histamine, steep increase at the doses of 10-2-101
μ g, thereafter a maximum plateau value of O. 206±
0.032 cc/gm were observed. The actual extravasated
or transudated plasma volumes can be calculated by
‘{}btaining the differences of clearance values of control
.site and inflammatory sites. The maximum plasma
"Volume extravasated was calculated as O. 17cc/gm which
is about 5. 7 times of the normal plasma volume.
4. From the point of view that dose-response curves
of total tissue water, extracellular fluid , intracellular
fluid and RISA clearance showed typical sigmoid cur·
ves, the inflammatory reactions induced by graded
doses of histamine were divided into 3 steps according
to their intensity and mechanism of changes. Each
step of changes was discussed.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 07 No.4 (1966)
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