동종 및 이종간조직반복주사에 의한 실험적 간염에 관한 연구
A Study on Experimental Hepatitis in Rats, Induced by Repeated Injection of Homologous and Heterologous Liver Tissue with Adjuvant

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손근찬; 이재구
Issue Date
서울대학교 의과대학
Seoul J Med 1969;10(3):75-93
A number of chronic inflammatory lesions of
unknown etiology in man have recently been understood
by the mechanism of so called autoimmune
disease and a series of experimental study using
animals have been extensively undertaken to reproduce
the lesions.
A study on experimental hepatitis in male rats
induced by ‘ repeated injections of homologous (rat)
and heterologous (rabbit) liver or heart tissue suspension
with Freund’ s complete adjuvant is made as
an attempt to produce immunological inflammatory
lesion of liver and clarify the nature and significance
of this type of lesion as a morphological view point,
concerning to a certain human chronic hepatic disorder,
the results obtained are as follows:
1) In the animal groups in which homologous liver
tissue suspension with adjuvant injected repeatedly,
considerably marked histopathological changes, namely
chronic interstitial hepatitis with focal necrosis were
observed in the most cases (about 92%). The lesions
consist of scattered· focal necrosis of liver cells and
infiltration of plasma cells, lymphocytes and histiocytcs
and occasionally of eosinophiles in the portal
spaces, associated with occasional mild fatty metamorphosis
and focal fibrosis.
2) Without adjuvant, the group in which homolo-gous liver tissue suspension only injected repeatedly
showed mild focal inflammatory changes in 50% of
3) The livers of the animal group in which heterologous
liver tissue suspension with adjuvant injected
repeatedly showed slight changes of the similar type,
but in far lesser degree as compared with former two
4) The livers of the group which received Fruend’ s
complete adjuvant only also showed some marked
proliferation of kupffer cells and some small round
cells infiltration wIth a few non-caseous granulomas.
5) The livers of the animal group which received
heart suspension (instead of liver suspension) with
Fruend’ s complete adjuvant showed no significant
inflammatory changes.
6) In other organ tissues , except some small round
cell infiltration, especially in the lung, spleen and
kidney and hyperplasia of some reticuloendothelial
system which is considered as the adjuvant effect,
no other particular changes were noted. Only, in
the brain , kidney and lungs, a few non-caseous
granulomatous lesions were noted.
7) The above results obtained by this experimentation
led the author to the conclusion, which says:
The hepatitic lesions induced in this experiment
might be considered as a chronic hypersensitivity
inflammatory changes; And, in some chronic inflammatory
liver diseases, the components of their own
hepatic tissue cells might be rendered antigenic by
some unknown reasons, and participates directly or
indirectly to their pathogenesis and disease process
through immunological mechanism, i. e. autoimmunity.
And from the above morphologic features , the
experimental hepatitc lesion presently described showed
many similarities to those of active chronic hepatitis
in man, so called ‘ Lupoid hepatitis".
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 10 No.3 (1969)
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