토끼 심장근육에서 부분정제한 H4-LDH의 특성에 관한 연구

Abstract

1. The H.-LDH was partially purified approximately 12 folds of its activity from the cardiac muscle of rabbits by means of ~salting-out with ammonium sulphate and of DEAE-cellulose column chromatography. 2. The activity of the H LDH was inhibited markedly by 0.02M pyruvate to 11% of its original activity 3. Sulfide ions. when added to the reaction system using lactate as the substrate. reduced slightly the inhibition caused by pyruvate. 4. Cysteine. on the other hand. lowered significantly the magnitude of pyruvate inhibition in its lower range of concentration. but showed its own inhibitory effe;t regardless of the pyruvate inhibition in its higher range of conce~tration. 5. The activity of H LDH. using lactate as the substrate was more inhibited by the addition of the sulfide ions than by the addition of 4 folds concentration of cysteine 6. When pyruvate was used as substrate. cysteine showed an apparently allosteric effect. while sulfide ions showed no particular effect different from the pyruvate induction itself.