Antibody-Based Surface Plasmon Resonance Detection of Intact Viral Pathogen
- Authors
- Baac, Hyoungwon; Hajos, Jozsef P.; Lee, Jennifer; Kim, Donghyun; Kim, Sung June; Shuler, Michael L.
- Issue Date
- 2006-07
- Publisher
- John Wiley & Sons
- Citation
- Biotechnol Bioeng 94:815-819
- Abstract
- Surface plasmon resonance (SPR) technique
was used to directly detect an intact form of insect
pathogen: the baculovirus, Autographa californica multiple
nuclear polyhedrosis virus (AcMNPV). An SPR sensor
chip with three bio-functional layerswasusedto detect the
intact AcMNPV: amine-reactive crosslinker with a disulfide
bond that chemisorbs to gold film, Protein A, and a
mouse IgG monoclonal antibody raised against a surface
protein of the target viral pathogen. A two-channel
(reference & test) micro-fluidic SPR system is used
for reliable measurement. Bio-specific response to the
AcMNPV is compared with the response for tobacco
mosaic virus (TMV) as control. Successive exposure of the
sensor chip to both viruses verifies a specific response to
AcMNPV. This serves as a prerequisite to the development
of a new type of viral pathogen detection sensors.
- ISSN
- 0006-3592
- Language
- English
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