SHERP

Antibody-Based Surface Plasmon Resonance Detection of Intact Viral Pathogen

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Authors
Baac, Hyoungwon; Hajos, Jozsef P.; Lee, Jennifer; Kim, Donghyun; Kim, Sung June; Shuler, Michael L.
Issue Date
2006-07
Publisher
John Wiley & Sons
Citation
Biotechnol Bioeng 94:815-819
Keywords
intact pathogensurface plasmon resonancebaculovirustobacco mosaic virus
Abstract
Surface plasmon resonance (SPR) technique
was used to directly detect an intact form of insect
pathogen: the baculovirus, Autographa californica multiple
nuclear polyhedrosis virus (AcMNPV). An SPR sensor
chip with three bio-functional layerswasusedto detect the
intact AcMNPV: amine-reactive crosslinker with a disulfide
bond that chemisorbs to gold film, Protein A, and a
mouse IgG monoclonal antibody raised against a surface
protein of the target viral pathogen. A two-channel
(reference & test) micro-fluidic SPR system is used
for reliable measurement. Bio-specific response to the
AcMNPV is compared with the response for tobacco
mosaic virus (TMV) as control. Successive exposure of the
sensor chip to both viruses verifies a specific response to
AcMNPV. This serves as a prerequisite to the development
of a new type of viral pathogen detection sensors.
ISSN
0006-3592
Language
English
URI
http://hdl.handle.net/10371/9377
DOI
https://doi.org/10.1002/bit.20882
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Journal Papers (저널논문_전기·정보공학부)
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