Determination of Glycogen Turnover Rates of the Liver, Cardiac Muscle and Skeletal Muscle of the Dog by" C^14-Glucose Dilution Methnd"

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Rhee, Sang Don; Nam, Kee Yong
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서울대학교 의과대학
Seoul J Med 1960;1(1):81-86
Several series of exreriments by Stetten, et aI.,
1.2) present current concepts of glycogen turnover in
the amimal body. In their earlier study , the turnover
rate of glycogen in vivo was estimated from
the measurement of stably bound deuterium appearing
in glycogen; the DzO concentration in the body
water was maintained by a DZO conatant infusion
method. This study using the rat showed that
deuterium concentration of liver and carcass
glycogen increased with time. Assuming the
glycogen turnover to be metabolically homogenous,
the turnover rates for liver glycogen and carcass
glycogen were calculated to be 68% and 1 % per
day, respectively. In subsequent experiments
employing C14_glucose. however. Stetten and Stetten2•
3) reported that peripheral tiers of glycogen
degraded enzymatically show more radioactivity
than the -less acce ssible limit dextrin within the
core of the glycogen molecules. These results
support the heterogeneity of glycogen turnover. The
earlier calculation by D떼 based on the assumption
of homogeneity must be revised.
Lorber. et aI.; ) postulated in their experiment
that blood sugar must be the principal precursor
for cardiac glycogen and that the incorporation of
labeled carbon into cardiac glycogen in the intact
amimal must be secondarγ to the formation of blα펴
sugar from administered labeled compounds. On the
basis of this assumption , Rhee. et aI5) . calculated the
turnover rate of the cardiac glycogen in the isolated
dog heart using the CJ4-glucose constant,infusion
method. In this experiment, the concentration
of CJ4-glucose and blood sugar in the perfusion system
was maintained constant by secondary injection
of additional labeled glucose. The reported turnover
rate was 6.4% per hour for cardiac glycogen which
was much higher than earlier reported values measured
by D잉 in liver and carcass glycogen in rats.
The quantitative measurement of the glycogen
turnover rate by tracer techniques has always inco
rporated a constant tracer infusion method for assuring
the steady incorporation of the tracers from
the blood into tissue glycogen This technique
requires detrmination of the characteristic disappearance
of a single injection of the tracer to calculate
the regulation of the infusion so as to maintain a
constant concentration of the tracer during the
experiment. Even so. it is difficult to maintain a
conatant concentration. Data from the single injection
method has in the past represented only qualitative
aspects of glycogen turnover. This paper
presents, however, the quantitative calculation of
the turnover rate after a single injection of 04
glucose; Fick’s principle as utilized in the indicator
dilution method" for determining cardiac output is
applied. This method is referred to as the “o‘glucose
dilution method".
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 01 No.1 (1960)
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