정상견의 C^14-포도당 대사

Abstract

With the aid of CJ4-labeled glucose. the metabolism of glucose in the intact dog was investigated in eight animals. C1Lglucose was administered in a single initial dose, specific activity of plasma glucose was allowed to decrease thereafter. On the basis of the S.A.-time curve of plasma glucose in wl>ich S.A. of plasma glucose decreased exponentially with time showing a kinetic characteristics of steady state of the glucose metabolism, the followiug data were determined. 1) The glucose pool of intact dog was calculated by dilution principle• obtaining ratio of total counts injected and zero time specific activity of plasma glucose, isotope the mean value of glucose pool was427.1 mg/kg. Assuming glucose level in the glucose pool was same with that of plasma, the distribution space of glucose in the body was calculated as an average of 40.7% of body weight. 2) Turnover rate and half time(t상 of the glucose pool were calculated by the first order kinetic equation obtaining the slope of the S.A.-time curve of plasma glucose. Average turnover rate of the glucose pool was 208.5mg/hr/kg of body weight and half time was 1.03 hour. 3) The percentage of respiratory C02 drived from plasma glucose, which was calculated by obtaining ratio of zero time S.A. of plasma glucose to respiratory C02 was 31.8% of the total C02 output. 4) Average value of 18.7% of glucose disappeared from plasma was oxidated to respiratory C02. From these data , it seems that minor portion of the disappeared glucose participated iu furnishing oxidative energy. 5) S.A. of glycogen isolated from liver. skeletal and cardiac muscle was measured and turnover rates of glycogens were compared qualitatively each other. Livcr glycogen was metabolically more active than ske· letal and cardiac glycogen.